Detailed information of TA system TA189768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 643727..644403
• Replicon: chromosome
• Accession: NZ_CP069077
• Organism: Mycobacterium tuberculosis strain N0072

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: JN135_RS02890
• Protein ID: WP_003898500.1
• Coordinates: 643727..644140 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: JN135_RS02895
• Protein ID: WP_003402915.1
• Coordinates: 644137..644403 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN135_RS02860	639072..639374	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
JN135_RS02865	639434..639712	-	279	WP_003402901.1	hypothetical protein	-
JN135_RS02870	639709..640962	-	1254	WP_244736122.1	inorganic phosphate transporter	-
JN135_RS02875	641082..641468	-	387	WP_003402907.1	VOC family protein	-
JN135_RS02880	641531..642415	-	885	WP_003402909.1	SDR family oxidoreductase	-
JN135_RS02885	642511..643413	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
JN135_RS02890	643727..644140	-	414	WP_003898500.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN135_RS02895	644137..644403	-	267	WP_003402915.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JN135_RS02900	644595..646310	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
JN135_RS02905	646388..647992	+	1605	WP_003903056.1	amidohydrolase	-
JN135_RS02910	647989..648969	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T189768 WP_003898500.1 NZ_CP069077:c644140-643727 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T189768 NZ_CP090989:2005110-2005298 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT189768 WP_003402915.1 NZ_CP069077:c644403-644137 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT189768 NZ_CP090989:2004828-2005085 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAATTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0