Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3125716..3126320 Replicon chromosome
Accession NZ_CP069076
Organism Mycobacterium tuberculosis strain N0031

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag JN136_RS14900 Protein ID WP_003414492.1
Coordinates 3125716..3126108 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag JN136_RS14905 Protein ID WP_003414495.1
Coordinates 3126105..3126320 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN136_RS14870 3120866..3121654 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
JN136_RS14875 3121988..3122533 - 546 WP_014584866.1 DUF1802 family protein -
JN136_RS14880 3122805..3123689 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
JN136_RS14885 3123692..3124579 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
JN136_RS14890 3124884..3125429 - 546 WP_014584866.1 DUF1802 family protein -
JN136_RS14895 3125426..3125695 - 270 WP_003912856.1 DUF2277 family protein -
JN136_RS14900 3125716..3126108 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
JN136_RS14905 3126105..3126320 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JN136_RS14910 3126367..3127116 + 750 WP_003414497.1 enoyl-CoA hydratase -
JN136_RS14915 3127195..3128277 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
JN136_RS14920 3128270..3129580 - 1311 WP_003901476.1 ABC transporter substrate-binding protein -
JN136_RS14925 3129583..3130410 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
JN136_RS14930 3130407..3131318 - 912 WP_003901477.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T189755 WP_003414492.1 NZ_CP069076:c3126108-3125716 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T189755 NZ_CP090977:473439-473789 [Planococcus sp. 107-1]
TTGATTGTAAAACGTGGTGACGTTTTTTTTGCAGAACTATCACCAGTTGTCGGGTCCGAACAAGGTGGGACCCGTCCGGT
GCTAGTGATTCAAAATGATATAGGAAATCGCTTTAGTCCCACTGTCATCATCGCGGCGATTACTGCACAGATTCAAAAAG
CGAAATTACCGACTCACGTTGAAATCAACGCTAAGAAATATGGCTTTGAGCGTGACTCTGTTATCTTGCTTGAACAATTG
CGCACGATTGATAAGTCGCGGCTGACAGATAAGATTACACAGCTGGATGAAACCTTGATGGAAAAAGTGGATGAAGCTTT
GGAAATCAGTGTGGGTCTTGTGAAGTTTTAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT189755 WP_003414495.1 NZ_CP069076:c3126320-3126105 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT189755 NZ_CP090977:473150-473434 [Planococcus sp. 107-1]
GTGTACGGGAAGTCAAAGACAAAAGAAGTGGTTGTGAAACTGCCGAAGCAAATGATCTCAGAATTGACTGCGCATTCAGA
TGAACAAATTCAAGAACGTGGTGATTATGTTTATGTTTCGACCCAACGGGTAACGAAGAACATATACGATTCACATCATA
TTCGGGAAGCAATGATCAAAGGCTACGTGGAGATGTCACAAATCAATCTTTCAATCGCTTGTGAATGTTCTCATGCAGAA
TACGAAGCGGAGCACACGACCGTGCGACTCGTAAGCGGGGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References