Detailed information of TA system TA189753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3096416..3096986
• Replicon: chromosome
• Accession: NZ_CP069076
• Organism: Mycobacterium tuberculosis strain N0031

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: JN136_RS14750
• Protein ID: WP_003414166.1
• Coordinates: 3096416..3096772 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: JN136_RS14755
• Protein ID: WP_003901465.1
• Coordinates: 3096756..3096986 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN136_RS14730	3091868..3093556	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
JN136_RS14735	3093560..3093886	-	327	WP_003414157.1	hypothetical protein	-
JN136_RS14740	3094059..3094646	+	588	WP_003914429.1	DUF3558 family protein	-
JN136_RS14745	3094665..3096314	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
JN136_RS14750	3096416..3096772	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
JN136_RS14755	3096756..3096986	-	231	WP_003901465.1	type II toxin-antitoxin system antitoxin MazE9	Antitoxin
JN136_RS14760	3097029..3098072	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
JN136_RS14765	3098071..3098538	+	468	WP_003414177.1	DUF1778 domain-containing protein	-
JN136_RS14770	3098714..3098968	-	255	WP_003917684.1	hypothetical protein	-
JN136_RS14775	3099116..3099520	+	405	WP_003414181.1	hypothetical protein	-
JN136_RS14780	3099517..3099708	+	192	WP_003414184.1	hypothetical protein	-
JN136_RS14785	3099925..3100185	+	261	Protein_2919	transposase	-
JN136_RS14790	3101295..3101552	+	258	WP_003899489.1	hypothetical protein	-
JN136_RS14795	3101657..3101968	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T189753 WP_003414166.1 NZ_CP069076:c3096772-3096416 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T189753 NZ_CP090968:3244950-3245483 [Edwardsiella piscicida]
ATGAATAGTGGGACAGAGTTTAAGGAGCTGAGTAAAGCTGATCACGACAGAGATTCTTTCGACTGCGGCGAAATAGAGCT
AAACAATTTTATTAAAAATTTCGCGTTAAAACACATGCGAGTGGGCATCAGTAAGACAATGGTTTTACCTGCTACTGATG
CCCTACCCAATAACAAACATCCCATCTGTGCTTTCTATACCGTAGCCCCAAGTTCAATTACCCGAAACTCACTCCCGGAC
GATCTAGGGAAGAAACTACCTCACTACCCTGTTCCCGTTTTCCTGCTTGCTCAGCTATCTGTTCACATTGACAATCAGGG
GGATGGATTAGGGAAAATAACGCTAATAAAAGCATTAGAGTATCTATGGAATGTTAACTACTTCTTACACGCTTATGCTG
TGGTAGTGGATTGTTTAAATGACAAGGCGGAGAGTTTCTACCGCAAGTATGGGTTCGAGACGCTATGCTCTTATGACGGT
CGCGTGAGGCTATTTTTACCCATGAAGACGGTAGCAGGACTGTTCTCTGACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT189753 WP_003901465.1 NZ_CP069076:c3096986-3096756 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT189753 NZ_CP090968:3244684-3244953 [Edwardsiella piscicida]
ATGGCTACAGCTCGCTTAGACCTGCGGTTAGACCCAGAAATCAAAGCGAAAGCAGAGAAAGCATCCGCTCTGCTCGGTTT
AAAAACTCTAACGGAATATATCGTTCATATTGTTGATAATGATGCAAAGAGAGTAATCGCTGCGCATGAAAGCATGGCTC
TACCAAACGACATTTTTGACCAATTTATGAATGCTTGTGAGCAGGCAAAAGAGCCGAATTCTGCCCTGAAATCAGCCCTA
TCATTTACTCGTGAAAAGGGCATTAAATGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• PDB: 7DU5
• PDB: 6KYT
• AlphaFold DB: A0A7U4FBW3