Detailed information of TA system TA189751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3056419..3057098
• Replicon: chromosome
• Accession: NZ_CP069076
• Organism: Mycobacterium tuberculosis strain N0031

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JN136_RS14520
• Protein ID: WP_003414059.1
• Coordinates: 3056419..3056835 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN136_RS14525
• Protein ID: WP_003414061.1
• Coordinates: 3056832..3057098 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN136_RS14495	3052471..3053373	-	903	WP_003901462.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN136_RS14500	3053442..3054194	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN136_RS14510	3054438..3054713	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN136_RS14515	3054710..3056332	-	1623	WP_003414057.1	class I SAM-dependent DNA methyltransferase	-
JN136_RS14520	3056419..3056835	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JN136_RS14525	3056832..3057098	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN136_RS14530	3057124..3057519	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JN136_RS14535	3057516..3057785	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN136_RS14540	3057795..3058889	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
JN136_RS14545	3058886..3059305	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN136_RS14550	3059304..3059378	+	75	Protein_2872	hypothetical protein	-
JN136_RS14555	3059379..3059858	-	480	WP_003414073.1	dihydrofolate reductase	-
JN136_RS14560	3059929..3060729	-	801	WP_003911953.1	thymidylate synthase	-
JN136_RS14565	3060885..3061622	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T189751 WP_003414059.1 NZ_CP069076:c3056835-3056419 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T189751 NZ_CP090968:c3093149-3092733 [Edwardsiella piscicida]
GTGGTCCTTTGGCGCTGTAATGTTCACGTCTCCTGGCGTACCCAGGTGATGTTTCTGCTGGGGCACGGCGCGCTGGTACT
GGCGATCCTGCTGGCGCCCTGGCCGGAAAACTACAGCGTATTCTGGCTGATTCTGCTGGTGGCGGTGATCTTTGAGTGTA
TCCGCAGCCAGCGCCGGATTGCGCGTAACCGCGGTGAGCTGCAGCTGCTGGCGCCGCAGGTGTGGCACTGGCAGCTGCGC
GAGTGGCGCCTGGCCCGACGCCCTTGGGTTAGCGATCTGGGGGCGCTGCTGATCCTGCAGAGTACCCATGGTGCGCCGTG
CCGCCGCCGTCTGTGGCTGGCGGCCGACAGCATGAGCCGCGAAGAGTGGGGGCAGCTGCGCCGCACGCTGCTCGATGCGG
GCGACGATCGCGCATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189751 WP_003414061.1 NZ_CP069076:c3057098-3056832 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189751 NZ_CP090968:c3093396-3093130 [Edwardsiella piscicida]
ATGGATATCAATAATAAAGCGCGGGTTCACTGGGCGTGCCGCCGCGGAATGCGCGAGCTGGATATCGCCATCATGCCTTT
CTTTGAACATGAGTATGATGCGCTTAACGATGCCGATAAACAGACCTTTGTCCGCCTGCTGGCCTGTGACGATCCTGAGC
TATTTAAATGGCTGATGAACCAGGGTAAGCCGGATGACGCCGATCTGGCCCGCATGATTACGCTGATTCAGACGCGAAAT
AAAGAACGTGGTCCTTTGGCGCTGTAA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64