Detailed information of TA system TA189749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2917505..2918219
• Replicon: chromosome
• Accession: NZ_CP069076
• Organism: Mycobacterium tuberculosis strain N0031

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: JN136_RS13695
• Protein ID: WP_003413460.1
• Coordinates: 2917779..2918219 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: JN136_RS13690
• Protein ID: WP_003413456.1
• Coordinates: 2917505..2917792 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN136_RS13655	2912927..2913172	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN136_RS13660	2913169..2913573	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
JN136_RS13665	2913790..2914410	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
JN136_RS13670	2914421..2914915	+	495	WP_003413444.1	DUF2617 family protein	-
JN136_RS13675	2914912..2915343	+	432	WP_003899390.1	DUF4247 domain-containing protein	-
JN136_RS13680	2915368..2915826	+	459	WP_003413451.1	DUF350 domain-containing protein	-
JN136_RS13685	2915823..2917394	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
JN136_RS13690	2917505..2917792	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
JN136_RS13695	2917779..2918219	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN136_RS13700	2918240..2918995	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
JN136_RS13705	2919128..2919724	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
JN136_RS13710	2919732..2920577	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
JN136_RS13715	2920606..2921505	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
JN136_RS13720	2921633..2922307	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T189749 WP_003413460.1 NZ_CP069076:2917779-2918219 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T189749 NZ_CP090968:c1727664-1727335 [Edwardsiella piscicida]
ATGAACTACACTATTGAGTACTACAGCGAAGAGGTTCGGCTTGAGGTCGATCAGTTGCCAATGGGTATGCGGGTTCGATA
CCAGCATCTCGTTGAACGCATGGAAATCTACGGTAGTAATCTCGGAGAACCACACACAAGCCCTTTTGGCGATGGGCTTT
TTGAGCTTCGAATCAAAGGCAGTGATGGCATCGCCCGCGTGTTTTACTGCACTCTTACCGGAAAACGTATCGTCATGCTG
CACAGCTTCATCAAGAAAACCCAGAAGACGCCAAGCGCTGAACGTAAGAAGGCTGAAACCAGAATGAAGGAGGTAAAACA
TGGCTGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT189749 WP_003413456.1 NZ_CP069076:2917505-2917792 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT189749 NZ_CP090968:c1727345-1727037 [Edwardsiella piscicida]
ATGGCTGGTAAACGCACCCCCCCCACGATGACGCATGATGAAATGGCTGCAAAATGGATGGAAGACCCGGCATTTAAAGC
AGAATACGATGCCATCGAAGAGGAATACGCCCTACTCGATGAAATGCTCGCCGCCCGCAAAAACGCGGGGCTGACCCAGG
CGCAGGTCGCCGAACGTATGGGAACCAAGCCTACAGCGATCACTCGCATGGAAAGTAATTTAGCCTCCGGCCAAAGCGGG
CCATCATTCGCCACGCTGAAAAAATTTGCACACGCGACCGGCAAGAAACTGCAGATCCGGTTTGTCTGA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8