Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2857165..2857802 Replicon chromosome
Accession NZ_CP069076
Organism Mycobacterium tuberculosis strain N0031

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN136_RS13430 Protein ID WP_003413180.1
Coordinates 2857165..2857560 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN136_RS13435 Protein ID WP_003413183.1
Coordinates 2857557..2857802 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN136_RS13390 2852568..2853779 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
JN136_RS13395 2853906..2854565 + 660 WP_003900846.1 LppA family lipoprotein -
JN136_RS13400 2854562..2855224 + 663 WP_003901421.1 LppA family lipoprotein -
JN136_RS13405 2855221..2855499 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN136_RS13410 2855592..2856005 + 414 WP_003413164.1 PIN domain nuclease -
JN136_RS13415 2856044..2856301 + 258 WP_003413167.1 CopG family transcriptional regulator -
JN136_RS13420 2856298..2856675 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN136_RS13425 2856691..2857065 - 375 WP_003413177.1 hypothetical protein -
JN136_RS13430 2857165..2857560 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN136_RS13435 2857557..2857802 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN136_RS13440 2858213..2858632 - 420 WP_003413190.1 A24 family peptidase -
JN136_RS13445 2858644..2859452 - 809 Protein_2656 shikimate dehydrogenase -
JN136_RS13450 2859449..2860702 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN136_RS13455 2860695..2861207 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189748 WP_003413180.1 NZ_CP069076:c2857560-2857165 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189748 NZ_CP090968:c1098609-1098406 [Edwardsiella piscicida]
ATGACCAAGATCGATTATCTGATGAAGCTGCGTAAATGCACGACGCTGGATACTCTAGAACGCGTGATCGAAAAGAATAA
ATACGAACTTTCAGATGATGAATTAGAAATTTTTTATTCTGCCGCAGATCATCGTCTGGCCGAATTAACTATGAATAAGC
TATACGACAAAATTCCAGCAGAAGTCTGGCAATACGTCCGCTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189748 WP_003413183.1 NZ_CP069076:c2857802-2857557 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189748 NZ_CP090968:c1098998-1098630 [Edwardsiella piscicida]
ATGGATGAGTACACTTCTTATCAGCATGACATCGCAGAGTTAAAATACCTGTGTGATTCTCTGTATCATCAGGGAATAGA
TGTCCTGGGAGAGAGCCATCACGGCTGGGTCAGCGACCCGACGGCAAAGGTAAATCTACAGCTTAATGAGCTGATTGAGC
ATATTGCCAGCGTCGCGCAATCATTTAAGATCAAATATCCGCATCATTCGGATTTAGCCGAAATATTGGATGATTATCTG
GATGAAACCTATGCCCTGTTTGGCGCCTATAGCGTCAGTGAAACGGCGCTCCGGCACTGGCTGCGCACCAAGCGGCGGGT
GGCATACTGCCTGGCGCATGAAAAGCGCAACGCTACCTTACATGTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References