Detailed information of TA system TA189745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2841705..2842345
• Replicon: chromosome
• Accession: NZ_CP069076
• Organism: Mycobacterium tuberculosis strain N0031

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JN136_RS13325
• Protein ID: WP_003412970.1
• Coordinates: 2841705..2842124 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JN136_RS13330
• Protein ID: WP_003412975.1
• Coordinates: 2842121..2842345 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN136_RS13295	2837295..2838017	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JN136_RS13300	2838534..2838756	+	223	Protein_2627	type II toxin-antitoxin system VapB family antitoxin	-
JN136_RS13305	2838753..2839154	+	402	WP_003412963.1	PIN domain-containing protein	-
JN136_RS13310	2839189..2840109	-	921	WP_003412965.1	restriction endonuclease	-
JN136_RS13315	2840450..2840656	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN136_RS13320	2840754..2841704	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
JN136_RS13325	2841705..2842124	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN136_RS13330	2842121..2842345	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JN136_RS13335	2842376..2845219	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN136_RS13340	2845291..2845692	-	402	WP_003412981.1	hypothetical protein	-
JN136_RS13345	2845692..2846162	-	471	WP_003899364.1	transcription antitermination factor NusB	-
JN136_RS13350	2846165..2846728	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189745 WP_003412970.1 NZ_CP069076:c2842124-2841705 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189745 NZ_CP090967:c3095050-3094634 [Edwardsiella piscicida]
GTGGTCCTTTGGCGCTGTAATGTTCACGTCTCCTGGCGTACCCAGGTGATGTTTCTGCTGGGGCACGGCGCGCTGGTACT
GGCGATCCTGCTGGCGCCCTGGCCGGAAAACTACAGCGTATTCTGGCTGATTCTGCTGGTGGCGGTGATCTTTGAGTGTA
TCCGCAGCCAGCGCCGGATTGCGCGTAACCGCGGTGAGCTGCAGCTGCTGGCGCCGCAGGTGTGGCACTGGCAGCTGCGC
GAGTGGCGCCTGGCCCGACGCCCTTGGGTTAGCGATCTGGGGGCGCTGCTGATCCTGCAGAGTACCCATGGTGCGCCGTG
CCGCCGCCGTCTGTGGCTGGCGGCCGACAGCATGAGCCGCGAAGAGTGGGGGCAGCTGCGCCGCACGCTGCTCGATGCGG
GCGACGATCGCGCATAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT189745 WP_003412975.1 NZ_CP069076:c2842345-2842121 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189745 NZ_CP090967:c3095297-3095031 [Edwardsiella piscicida]
ATGGATATCAATAATAAAGCGCGGGTTCACTGGGCGTGCCGCCGCGGAATGCGCGAGCTGGATATCGCCATCATGCCTTT
CTTTGAACATGAGTATGATGCGCTTAACGATGCCGATAAACAGACCTTTGTCCGCCTGCTGGCCTGTGACGATCCTGAGC
TATTTAAATGGCTGATGAACCAGGGTAAGCCGGATGACGCCGATCTGGCCCGCATGATTACGCTGATTCAGACGCGAAAT
AAAGAACGTGGTCCTTTGGCGCTGTAA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09