Detailed information of TA system TA189733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2183223..2183926
• Replicon: chromosome
• Accession: NZ_CP069076
• Organism: Mycobacterium tuberculosis strain N0031

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JN136_RS10155
• Protein ID: WP_003409778.1
• Coordinates: 2183223..2183552 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JN136_RS10160
• Protein ID: WP_003409780.1
• Coordinates: 2183549..2183926 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN136_RS10135	2179606..2180676	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
JN136_RS10140	2180673..2181188	+	516	WP_003409718.1	flavin reductase family protein	-
JN136_RS10145	2181185..2182246	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JN136_RS10150	2182243..2183013	+	771	WP_003409775.1	SDR family oxidoreductase	-
JN136_RS10155	2183223..2183552	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JN136_RS10160	2183549..2183926	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JN136_RS10165	2183923..2184513	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
JN136_RS10170	2184568..2185932	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
JN136_RS10175	2186087..2186539	-	453	WP_003899095.1	lipoprotein	-
JN136_RS10180	2186603..2187004	+	402	WP_003409869.1	hypothetical protein	-
JN136_RS10185	2186997..2187179	-	183	WP_003409870.1	hypothetical protein	-
JN136_RS10190	2187293..2187643	-	351	WP_003409871.1	hypothetical protein	-
JN136_RS10195	2187654..2188556	-	903	WP_003899097.1	hypothetical protein	-
JN136_RS10200	2188577..2188768	-	192	WP_003901295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T189733 WP_003409778.1 NZ_CP069076:c2183552-2183223 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T189733 NZ_CP090959:3543897-3544148 [Simiduia sp. 21SJ11W-1]
ATGGACGCCATAAGCTACACAGCAGCCCGAGCAAACCTTGCCAAAACCATGGAGCGGGTCTGTAACGACCACTCTCCAGT
GATAATTACCAGAAAGAGCGAGGCACCCGTGGTAATGGTCTCCCTCGAGGACTACCAGGCTATGGAGGAAACCGCCTACC
TTCTGCGCTCTCCTGCAAACGCTAGACATCTGCTTGAGTCTATAGCTGAGCTCGAGGCAGGAAAAGGCTCTGAGAGAGAG
CTAATTGAATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT189733 WP_003409780.1 NZ_CP069076:c2183926-2183549 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT189733 NZ_CP090959:3544145-3544405 [Simiduia sp. 21SJ11W-1]
ATGAAGTTAACTTTCTCCTCCAGGGCTTGGGAAGAGTATCTATATTGGCAGAAAACAGACAAAGCCATGGTCAAAAGGAT
TAACGCATTGATCAAGGACACTTCCCGTAACCCATCTGAAGGGATAGGTAAGCCAGAGCCGCTAAAGCATGGCCTTTCTG
GCTATTGGTCTCGACGTATTAACGATGAGCACCGCTTCGTCTATAAAGCGTCCGATGATGGTCTTTTGATCGCACAGTTA
CGCTATCACTACGAGTACTAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8