Detailed information of TA system TA189725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1384583..1385271
• Replicon: chromosome
• Accession: NZ_CP069076
• Organism: Mycobacterium tuberculosis strain N0031

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: JN136_RS06605
• Protein ID: WP_003406304.1
• Coordinates: 1384840..1385271 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: JN136_RS06600
• Protein ID: WP_003406302.1
• Coordinates: 1384583..1384843 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN136_RS06580	1380160..1380984	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
JN136_RS06585	1380989..1382170	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
JN136_RS06590	1382247..1383347	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
JN136_RS06595	1383518..1384507	+	990	WP_003406301.1	malate dehydrogenase	-
JN136_RS06600	1384583..1384843	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
JN136_RS06605	1384840..1385271	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN136_RS06610	1385294..1386876	-	1583	Protein_1305	PE family protein	-
JN136_RS06615	1387056..1387916	+	861	WP_003901118.1	glycine betaine ABC transporter substrate-binding protein	-
JN136_RS06620	1387997..1388827	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
JN136_RS06625	1388884..1389177	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
JN136_RS06630	1389174..1389443	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T189725 WP_003406304.1 NZ_CP069076:1384840-1385271 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T189725 NZ_CP090953:c1621584-1621261 [Sphingomonas sp. S2-65]
ATGAAGTTCCTGGTCGACGCGCAACTGCCGCCCGCTTTGTGCGGGTGGTTGCGCGAGCGTGGCCATGAGGCGGAGCATGT
GTTCGAGATCGGCATGGTGGCAGCGAGCGACGCCGATATTGCGGCTCGGGCTGAAGCGGACGGCGCGGTGCTGGTCAGCA
AGGATGAGGATTTCGTCACGCTGCGACTGCCCGATCGGTTTGCGTTCGTGTGGCTGCGCTGTGGGAATGCGACGAACCGA
GCGCTACTTGCTTGGTTGGAGGCCCGCTGGGATCAGGTGCAACGATTGCTGGCTGCTTCTGAGAGGTATATCGAAGTCAT
ATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT189725 WP_003406302.1 NZ_CP069076:1384583-1384843 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT189725 NZ_CP090953:c1621814-1621581 [Sphingomonas sp. S2-65]
ATGACGCTGGCTGGCTTTCCCCGCATCGCAGTCGATCCGCTGGTGTGCGGAGGCAGGCCAATCGTGGCCGGTACCCGTGT
TCGGGTGACCGATGTGCTCGAGATGCTGGCGGGCGGCGCCAGCCCGGGCGAGATCGTCGGGGACTTTCCCTACCTCAGCG
AGGAAGATGTGCGGGCGGTGCTTGCCTATGCCGCGCGGCAGGCTGACCATCCGGTAGTGCTCGCCGCCGAATGA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6