Detailed information of TA system TA189724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1238426..1238994
• Replicon: chromosome
• Accession: NZ_CP069076
• Organism: Mycobacterium tuberculosis strain N0031

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JN136_RS05930
• Protein ID: WP_003405865.1
• Coordinates: 1238620..1238994 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN136_RS05925
• Protein ID: WP_003405863.1
• Coordinates: 1238426..1238623 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN136_RS05905	1234467..1235105	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN136_RS05910	1235195..1236202	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN136_RS05915	1236219..1237202	-	984	WP_003898731.1	hypothetical protein	-
JN136_RS05920	1237265..1238338	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JN136_RS05925	1238426..1238623	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN136_RS05930	1238620..1238994	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN136_RS05935	1239197..1239895	+	699	WP_003898733.1	hypothetical protein	-
JN136_RS05940	1240013..1240198	+	186	WP_003901093.1	hypothetical protein	-
JN136_RS05945	1240125..1240400	-	276	WP_003405867.1	hypothetical protein	-
JN136_RS05950	1240643..1240966	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN136_RS05955	1240981..1241679	-	699	WP_031646953.1	hypothetical protein	-
JN136_RS05960	1241713..1241922	+	210	WP_003911400.1	hypothetical protein	-
JN136_RS05965	1241874..1242644	-	771	Protein_1175	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T189724 WP_003405865.1 NZ_CP069076:1238620-1238994 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189724 NZ_CP090942:c29631-29365 [Staphylococcus epidermidis]
ATGGCTAAATATAAAATCAACATTAAGAATTCAGCGAAGACAGATTTAAAGAAGATAAAACGTTCTCACTTACAAGAACA
ATTTTTGAAAATCGTAGAAATACTAAAATATGATCCATATCAACAAAGTCAATCCTTTGAAAAATTGCACCCTAAACATT
TAGGACGATACTCAAGACGTATCAATCATCAACATAGAGTTGTCTATACAATTGATGAGGAAAATAAAGAAGTATTAATA
TTAGCAACTTGGTCTCATTATGAATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189724 WP_003405863.1 NZ_CP069076:1238426-1238623 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189724 NZ_CP090942:c29888-29631 [Staphylococcus epidermidis]
ATGAAAAGTAAAACACCAACAGAAGCTCGTAAAGATTTTTATCAGTTATTAAAACGTGTGAATAATGACCATGAACCAGT
ATATATTGAAGGCAAATATGATGAGAGTAATGCTGTTATTATAGGCATGGAAGATTGGCGTAGCATTAATGAAACGATAT
ATCTTGAGGCTACAGGCACAATGGACAAAGTTAGAGAACGTGAGCATGACAACAGTGGTTATACAAACATAGATGATATT
GATTGGGCTAGACTTTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07