Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1021629..1022602 Replicon chromosome
Accession NZ_CP069076
Organism Mycobacterium tuberculosis strain N0031

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag JN136_RS04850 Protein ID WP_003404756.1
Coordinates 1022102..1022602 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID G0TFR4
Locus tag JN136_RS04845 Protein ID WP_003404754.1
Coordinates 1021629..1022105 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN136_RS04825 1017476..1018747 - 1272 WP_003404747.1 PPE family protein -
JN136_RS04830 1018762..1019061 - 300 WP_003404748.1 PE family protein -
JN136_RS04835 1019505..1021286 + 1782 WP_003404749.1 BCCT family transporter -
JN136_RS04840 1021286..1021540 + 255 WP_003898646.1 hypothetical protein -
JN136_RS04845 1021629..1022105 + 477 WP_003404754.1 DUF1778 domain-containing protein Antitoxin
JN136_RS04850 1022102..1022602 + 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
JN136_RS04860 1022915..1024234 - 1320 WP_003404757.1 IS256-like element IS1554 family transposase -
JN136_RS04865 1024522..1025103 + 582 WP_003404760.1 IS607-like element IS1535 family transposase -
JN136_RS04870 1025103..1026755 + 1653 WP_003404761.1 IS607 family element RNA-guided endonuclease TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(38-143)

Antitoxin

Vibrio phage ICP1, Orf50

(74-150)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T189721 WP_003404756.1 NZ_CP069076:1022102-1022602 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp

>T189721 NZ_CP090941:976630-976812 [Staphylococcus epidermidis]
ATGGCGAACAAAAAAGATTCCAAATTAAATTATCATGAAGAAGAAAACGCTATGGTTACTGACTTAGATGATTTAAAAGA
ATTAGGAAAAGAAATGGAACAGATTTCACAGGAAAATGATGAAGAAAAATTAAATCAAACTCATGATAACGAAGTTCGTT
CAGATTTTAAAAACAGTAAATAG

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17357.95 Da        Isoelectric Point: 11.2467

>AT189721 WP_003404754.1 NZ_CP069076:1021629-1022105 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGGSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp

>AT189721 NZ_CP090941:976007-976606 [Staphylococcus epidermidis]
ATGGCAATGAATTTTAAAGTATTTGACAATAGTGAAAAAGTAGCAGAATATACTGCAGATATTCTACGTAAGCAGTTTAA
TAATAATCCAACTACTATTGCAGGATTTCATTTGAGCAAAGAACATGCACCAGTGTTTGATGAACTTAAGAAAAATGTGG
AAAATCATGCAGTTGATTTCAGTCAAATAAATATTTTAGATTATGATGATAATCATTCTTATTATGAGGCTTTGGGTGTG
CCAACAGGTCAAATTTATTCCATTTCTTACGAAAATGATGCAATTGACTTTATCTCAGATAAGATTAAGACTAAAGAAAA
TAAAGGCAAACTGACAATGCAAGTCCTTACGATTGATGAAAATGGCAAACTTGACATTAGCGTTCGTCAAGGTTTGATGG
AAGCTAGAGAAATTTTCTTAGTTATTACTGGTGCTAATAAACGCGATATGGTAGAAAAACTTTACCGTGAAAATGGTAAG
ACTAGTTTTGAACCATCGGATTTAAAAGCACACCGTATGGTCAATGTTATCTTAGATAAAGAAGCGGCTGCAGGCTTACC
TGAAGACGTAAAAGAGTACTTCACTGCACGTTTTGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2

Antitoxin

Source ID Structure
AlphaFold DB G0TFR4

References