Detailed information of TA system TA189715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 749980..750613
• Replicon: chromosome
• Accession: NZ_CP069076
• Organism: Mycobacterium tuberculosis strain N0031

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQD6
• Locus tag: JN136_RS03445
• Protein ID: WP_003403365.1
• Coordinates: 749980..750363 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: JN136_RS03450
• Protein ID: WP_003403368.1
• Coordinates: 750458..750613 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN136_RS03420	745272..745808	+	537	WP_003403341.1	50S ribosomal protein L10	-
JN136_RS03425	745845..746237	+	393	WP_003900989.1	50S ribosomal protein L7/L12	-
JN136_RS03430	746230..746925	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
JN136_RS03435	746996..748501	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
JN136_RS03440	748582..749592	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
JN136_RS03445	749980..750363	-	384	WP_003403365.1	ribonuclease VapC6	Toxin
JN136_RS03450	750458..750613	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN136_RS03455	750689..751405	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
JN136_RS03460	751681..751989	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
JN136_RS03465	751976..752221	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
JN136_RS03470	752331..752768	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
JN136_RS03475	752765..753019	-	255	WP_003911263.1	antitoxin VapB7	-
JN136_RS03480	753133..755496	+	2364	WP_003900991.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13959.73 Da
• Isoelectric Point: 6.0803

>T189715 WP_003403365.1 NZ_CP069076:c750363-749980 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T189715 NZ_CP090932:137677-138147 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTTTTTTACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCTCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATTGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT189715 WP_003403368.1 NZ_CP069076:c750613-750458 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT189715 NZ_CP090932:137237-137680 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TQD6

Antitoxin

• AlphaFold DB: A0A7U4BSF8