Detailed information of TA system TA189699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3782507..3783036
• Replicon: chromosome
• Accession: NZ_CP069075
• Organism: Mycobacterium tuberculosis strain N0004

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: JN138_RS17815
• Protein ID: WP_003417760.1
• Coordinates: 3782779..3783036 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: JN138_RS17810
• Protein ID: WP_003417757.1
• Coordinates: 3782507..3782782 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN138_RS17775	3779080..3779769	-	690	WP_003917774.1	BBE domain-containing protein	-
JN138_RS17780	3779956..3780327	-	372	WP_003417739.1	FAD-binding protein	-
JN138_RS17785	3780225..3780443	-	219	WP_157132559.1	hypothetical protein	-
JN138_RS17790	3780470..3780730	-	261	WP_003417741.1	hypothetical protein	-
JN138_RS17795	3780845..3781234	+	390	WP_003417745.1	DUF732 domain-containing protein	-
JN138_RS17800	3781248..3781541	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
JN138_RS17805	3781538..3782383	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
JN138_RS17810	3782507..3782782	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
JN138_RS17815	3782779..3783036	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
JN138_RS17820	3783078..3784268	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
JN138_RS17825	3784385..3784752	+	368	Protein_3529	FHA domain-containing protein	-
JN138_RS17830	3784749..3785300	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
JN138_RS17835	3785307..3785888	-	582	WP_003417769.1	ATP/GTP-binding protein	-
JN138_RS17840	3785869..3786237	-	369	WP_003417772.1	DUF742 domain-containing protein	-
JN138_RS17845	3786215..3786607	-	393	WP_003417776.1	serine protease inhibitor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T189699 WP_003417760.1 NZ_CP069075:3782779-3783036 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T189699 NZ_CP090930:c4321095-4320787 [Salmonella enterica subsp. enterica serovar Agona]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGATGGGTTCTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT189699 WP_003417757.1 NZ_CP069075:3782507-3782782 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT189699 NZ_CP090930:c4321337-4321098 [Salmonella enterica subsp. enterica serovar Agona]
ATGTCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTAGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCTGCGGTGCTTACCGCTGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94