Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3190390..3191077 Replicon chromosome
Accession NZ_CP069075
Organism Mycobacterium tuberculosis strain N0004

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag JN138_RS15245 Protein ID WP_003414624.1
Coordinates 3190634..3191077 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag JN138_RS15240 Protein ID WP_003414620.1
Coordinates 3190390..3190647 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN138_RS15220 3185710..3186564 - 855 WP_244694680.1 GNAT family N-acetyltransferase -
JN138_RS15225 3186620..3187783 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
JN138_RS15230 3187800..3189014 - 1215 WP_003414610.1 zinc metalloprotease Rip -
JN138_RS15235 3189022..3190263 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
JN138_RS15240 3190390..3190647 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
JN138_RS15245 3190634..3191077 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
JN138_RS15250 3191157..3191819 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
JN138_RS15255 3191915..3192106 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
JN138_RS15260 3192438..3194186 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
JN138_RS15265 3194282..3194863 + 582 WP_003414644.1 fasciclin domain-containing protein -
JN138_RS15270 3194963..3195229 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T189696 WP_003414624.1 NZ_CP069075:3190634-3191077 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T189696 NZ_CP090930:3642622-3642840 [Salmonella enterica subsp. enterica serovar Agona]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT189696 WP_003414620.1 NZ_CP069075:3190390-3190647 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT189696 NZ_CP090930:3642220-3642594 [Salmonella enterica subsp. enterica serovar Agona]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References