Detailed information of TA system TA189690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3077551..3078230
• Replicon: chromosome
• Accession: NZ_CP069075
• Organism: Mycobacterium tuberculosis strain N0004

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JN138_RS14640
• Protein ID: WP_003414059.1
• Coordinates: 3077551..3077967 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN138_RS14645
• Protein ID: WP_003414061.1
• Coordinates: 3077964..3078230 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN138_RS14615	3073603..3074505	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN138_RS14620	3074574..3075326	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN138_RS14630	3075570..3075845	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN138_RS14635	3075842..3077464	-	1623	WP_015630537.1	class I SAM-dependent DNA methyltransferase	-
JN138_RS14640	3077551..3077967	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JN138_RS14645	3077964..3078230	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN138_RS14650	3078256..3078651	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JN138_RS14655	3078648..3078917	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN138_RS14660	3078927..3080021	-	1095	WP_015630539.1	restriction endonuclease subunit S	-
JN138_RS14665	3080018..3080437	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN138_RS14670	3080436..3080510	+	75	Protein_2902	hypothetical protein	-
JN138_RS14675	3080511..3080990	-	480	WP_003414073.1	dihydrofolate reductase	-
JN138_RS14680	3081061..3081861	-	801	WP_244694664.1	thymidylate synthase	-
JN138_RS14685	3082017..3082754	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T189690 WP_003414059.1 NZ_CP069075:c3077967-3077551 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T189690 NZ_CP090930:872969-873382 [Salmonella enterica subsp. enterica serovar Agona]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189690 WP_003414061.1 NZ_CP069075:c3078230-3077964 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189690 NZ_CP090930:872722-872988 [Salmonella enterica subsp. enterica serovar Agona]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64