Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2876853..2877490 Replicon chromosome
Accession NZ_CP069075
Organism Mycobacterium tuberculosis strain N0004

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN138_RS13540 Protein ID WP_003413180.1
Coordinates 2876853..2877248 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN138_RS13545 Protein ID WP_003413183.1
Coordinates 2877245..2877490 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN138_RS13500 2872256..2873467 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
JN138_RS13505 2873594..2874253 + 660 WP_003900846.1 LppA family lipoprotein -
JN138_RS13510 2874250..2874912 + 663 WP_023643170.1 LppA family lipoprotein -
JN138_RS13515 2874909..2875187 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN138_RS13520 2875280..2875693 + 414 WP_003413164.1 PIN domain nuclease -
JN138_RS13525 2875732..2875989 + 258 WP_003413167.1 CopG family transcriptional regulator -
JN138_RS13530 2875986..2876363 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN138_RS13535 2876379..2876753 - 375 WP_003413177.1 hypothetical protein -
JN138_RS13540 2876853..2877248 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN138_RS13545 2877245..2877490 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN138_RS13550 2877901..2878320 - 420 WP_003413190.1 A24 family peptidase -
JN138_RS13555 2878332..2879141 - 810 WP_003413193.1 shikimate dehydrogenase -
JN138_RS13560 2879138..2880391 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN138_RS13565 2880384..2880896 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189687 WP_003413180.1 NZ_CP069075:c2877248-2876853 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189687 NZ_CP090929:c4826566-4826255 [Salmonella enterica subsp. enterica serovar Agona]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189687 WP_003413183.1 NZ_CP069075:c2877490-2877245 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189687 NZ_CP090929:c4826254-4825964 [Salmonella enterica subsp. enterica serovar Agona]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGGTTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGGTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References