Detailed information of TA system TA189686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2875732..2876363
• Replicon: chromosome
• Accession: NZ_CP069075
• Organism: Mycobacterium tuberculosis strain N0004

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: JN138_RS13530
• Protein ID: WP_003413174.1
• Coordinates: 2875986..2876363 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: JN138_RS13525
• Protein ID: WP_003413167.1
• Coordinates: 2875732..2875989 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN138_RS13495	2871646..2871960	+	315	WP_009937839.1	hypothetical protein	-
JN138_RS13500	2872256..2873467	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
JN138_RS13505	2873594..2874253	+	660	WP_003900846.1	LppA family lipoprotein	-
JN138_RS13510	2874250..2874912	+	663	WP_023643170.1	LppA family lipoprotein	-
JN138_RS13515	2874909..2875187	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
JN138_RS13520	2875280..2875693	+	414	WP_003413164.1	PIN domain nuclease	-
JN138_RS13525	2875732..2875989	+	258	WP_003413167.1	CopG family transcriptional regulator	Antitoxin
JN138_RS13530	2875986..2876363	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN138_RS13535	2876379..2876753	-	375	WP_003413177.1	hypothetical protein	-
JN138_RS13540	2876853..2877248	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JN138_RS13545	2877245..2877490	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JN138_RS13550	2877901..2878320	-	420	WP_003413190.1	A24 family peptidase	-
JN138_RS13555	2878332..2879141	-	810	WP_003413193.1	shikimate dehydrogenase	-
JN138_RS13560	2879138..2880391	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
JN138_RS13565	2880384..2880896	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T189686 WP_003413174.1 NZ_CP069075:2875986-2876363 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T189686 NZ_CP090929:4364928-4365344 [Salmonella enterica subsp. enterica serovar Agona]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTCGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGCCGGTACGCCGATTGGATCAAACGATGCGGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT189686 WP_003413167.1 NZ_CP069075:2875732-2875989 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT189686 NZ_CP090929:4364701-4364931 [Salmonella enterica subsp. enterica serovar Agona]
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5