Detailed information of TA system TA189675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2228915..2229603
• Replicon: chromosome
• Accession: NZ_CP069075
• Organism: Mycobacterium tuberculosis strain N0004

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P0A653
• Locus tag: JN138_RS10470
• Protein ID: WP_003409958.1
• Coordinates: 2228915..2229334 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: JN138_RS10475
• Protein ID: WP_003409968.1
• Coordinates: 2229343..2229603 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN138_RS10450	2224418..2225258	+	841	Protein_2066	class I SAM-dependent methyltransferase	-
JN138_RS10455	2225221..2226666	-	1446	WP_003899115.1	APC family permease	-
JN138_RS10460	2226845..2227531	-	687	WP_003409954.1	immunoprotective protein Mpt64	-
JN138_RS10465	2227722..2228690	-	969	WP_003899116.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
JN138_RS10470	2228915..2229334	-	420	WP_003409958.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN138_RS10475	2229343..2229603	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN138_RS10480	2229746..2231422	+	1677	WP_003899118.1	PecA family PE domain-processing aspartic protease	-
JN138_RS10485	2231410..2232063	-	654	WP_003409976.1	cutinase Cfp21	-
JN138_RS10490	2232178..2232408	+	231	WP_003409980.1	DUF167 domain-containing protein	-
JN138_RS10495	2232493..2233404	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
JN138_RS10500	2233513..2234112	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14724.89 Da
• Isoelectric Point: 7.9535

>T189675 WP_003409958.1 NZ_CP069075:c2229334-2228915 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T189675 NZ_CP090929:210108-210593 [Salmonella enterica subsp. enterica serovar Agona]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTTGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGGGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT189675 WP_003409968.1 NZ_CP069075:c2229603-2229343 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT189675 NZ_CP090929:209833-210120 [Salmonella enterica subsp. enterica serovar Agona]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCGGGCTGCCGAGAATGTGATCC
TTGACCGCCGTATATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A829C4H9

Antitoxin

• AlphaFold DB: A0A829C483