Detailed information of TA system TA189674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 2203236..2203780
• Replicon: chromosome
• Accession: NZ_CP069075
• Organism: Mycobacterium tuberculosis strain N0004

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TLU9
• Locus tag: JN138_RS10320
• Protein ID: WP_003409896.1
• Coordinates: 2203236..2203532 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P67299
• Locus tag: JN138_RS10325
• Protein ID: WP_003409899.1
• Coordinates: 2203529..2203780 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN138_RS10265	2198269..2198619	-	351	WP_003409871.1	hypothetical protein	-
JN138_RS10270	2198630..2199532	-	903	WP_003899097.1	hypothetical protein	-
JN138_RS10275	2199553..2199744	-	192	WP_003409876.1	hypothetical protein	-
JN138_RS10280	2199745..2200041	-	297	WP_003409877.1	hypothetical protein	-
JN138_RS10285	2200281..2200496	+	216	WP_003409878.1	antitoxin	-
JN138_RS10290	2200493..2200804	+	312	WP_003409881.1	type II toxin-antitoxin system VapC family toxin	-
JN138_RS10295	2200778..2201299	-	522	WP_003904745.1	hypothetical protein	-
JN138_RS10300	2201274..2201651	+	378	WP_010886136.1	type II toxin-antitoxin system toxin HigB	-
JN138_RS10305	2201693..2202142	+	450	WP_003409886.1	type II toxin-antitoxin system antitoxin HigA	-
JN138_RS10310	2202139..2202684	+	546	WP_003409891.1	SecB-like chaperone	-
JN138_RS10315	2202573..2203187	-	615	WP_003901296.1	hypothetical protein	-
JN138_RS10320	2203236..2203532	-	297	WP_003409896.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JN138_RS10325	2203529..2203780	-	252	WP_003409899.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
JN138_RS10330	2203767..2204261	+	495	WP_003899099.1	hypothetical protein	-
JN138_RS10335	2204421..2204828	-	408	WP_003409913.1	type II toxin-antitoxin system VapC family toxin	-
JN138_RS10340	2204832..2205104	-	273	WP_003899100.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
JN138_RS10345	2205137..2206357	-	1221	WP_003409919.1	TetR family transcriptional regulator Mce3R	-
JN138_RS10350	2207256..2208053	+	798	WP_003899101.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Antitoxin ParD (2-80)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11268.64 Da
• Isoelectric Point: 6.8604

>T189674 WP_003409896.1 NZ_CP069075:c2203532-2203236 [Mycobacterium tuberculosis]
VSSRYLLSPAAQAHLEEIWDCTYDRWGVDQAEQYLRELQHAIDRAAANPRIGRACDEIRPGYRKLSAGSHTLFYRVTGEG
TIDVVRVLHQRMDVDRNL

Nucleotide

• Length: 297 bp

>T189674 NZ_CP090922:1962320-1962508 [Staphylococcus epidermidis]
ATGAGCTTACATTTCCAAATTTTGCTGTGGCTTTCTATCTTATTTATCATCGCAGGAACGATATTATTGGTAACAATGCT
TAAAACTAAAAAAGAAGAACGAAAAGAATCTTATCTAGGCTTTACTGTAATTTTCTTAATCTTTGGTTTTGCCATCTTAA
TTTATACTTTTATATTCGGAATTTTATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9207.17 Da
• Isoelectric Point: 7.0150

>AT189674 WP_003409899.1 NZ_CP069075:c2203780-2203529 [Mycobacterium tuberculosis]
VGKNTSFVLDEHYSAFIDGEIAAGRYRSASEVIRSALRLLEDRETQLRALREALEAGERSGSSTPFDFDGFLGRKRADAS
RGR

Nucleotide

• Length: 252 bp

>AT189674 NZ_CP090922:1962038-1962295 [Staphylococcus epidermidis]
ATGAAATTAAACAAAGCAAATATATTCAACTTGATATTCACCATTTTATTTTTTTCATTTAACATTCTCATAACCTATAA
CGCAAATATAGATTATAAGTTATGGTTAATACCTGGTTTAGCAATTTGTGGTTTTGCTCTTTTTTCAAGCTTGACCTTAG
TAATTATCTATAGTGATTTATTCAGTGAAATATTATTTTTTATCAACATTATCTTAGCACTGTATTACATTTATCCGATT
TTTTATGAATTTGTCTAA

Structures

Toxin

• AlphaFold DB: G0TLU9

Antitoxin

• AlphaFold DB: A0A7U4BUZ2