Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1952904..1953517 Replicon chromosome
Accession NZ_CP069075
Organism Mycobacterium tuberculosis strain N0004

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag JN138_RS09145 Protein ID WP_003408465.1
Coordinates 1952904..1953293 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag JN138_RS09150 Protein ID WP_003408469.1
Coordinates 1953290..1953517 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN138_RS09110 1948533..1949447 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
JN138_RS09115 1949450..1950280 + 831 WP_003408448.1 cyclase family protein -
JN138_RS09120 1950280..1950630 + 351 WP_003898986.1 cupin domain-containing protein -
JN138_RS09125 1950683..1951501 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
JN138_RS09130 1951515..1952294 + 780 WP_003408460.1 IclR family transcriptional regulator -
JN138_RS09140 1952725..1952856 + 132 Protein_1803 IS3 family transposase -
JN138_RS09145 1952904..1953293 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
JN138_RS09150 1953290..1953517 - 228 WP_003408469.1 antitoxin Antitoxin
JN138_RS09155 1953735..1955219 + 1485 WP_003408473.1 biotin carboxylase -
JN138_RS09160 1955216..1956463 + 1248 WP_003408476.1 serine hydrolase -
JN138_RS09165 1956506..1956925 - 420 WP_003408483.1 hypothetical protein -
JN138_RS09170 1956915..1957625 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T189670 WP_003408465.1 NZ_CP069075:c1953293-1952904 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T189670 NZ_CP090917:c26820-26482 [Staphylococcus epidermidis]
ATGAGTATCAAACAGTTTGATATTTACTATATTGACTTAGACCCAACTCGTGGCCGAGAAAAACGAAAAGTCAGACCATG
CTTAATCGTAAATAATCAAATGACAATCAATGGCACAAACTTCGTTTGGATATTGCCAATTACAAGTCGAGAAGTAAAAT
TCCCAACCGATATTAAAGTTAAAACTAAAAATGGATTAGTTACAGGTGTCATAGATACCATTCAGATACGTGCTTTAGAC
ATTAATGCCCGTTATCATAACTATAAAGATGAATTACAAGAAAACTTAAAAAGTGACGTATTAAAAGCAATACAGACGTA
TCTCAAACCAATATTATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT189670 WP_003408469.1 NZ_CP069075:c1953517-1953290 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT189670 NZ_CP090917:c27065-26817 [Staphylococcus epidermidis]
ATGAATAAAACATCTGAACGAATCTTCAAAAGCGGTAACAGCAAAGCAATTAGTTTAGGAAAACAAACAATCCAACTCAC
TGATTTTGAAGTAGGGGATAAAGTTGAAGTTCAACAAGTTGATGGTGGTCTATTCATCACAAAAAAAGAACAATCTATTG
AAGATGAAATCACAAACTTTTTCAAAAATGGTGGTAAATACACTGAACCTGAAATCGACTTTGGCGAGAGTGTAGGTCGC
GAAATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References