Detailed information of TA system TA189669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1770591..1771204
• Replicon: chromosome
• Accession: NZ_CP069075
• Organism: Mycobacterium tuberculosis strain N0004

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: JN138_RS08325
• Protein ID: WP_003407786.1
• Coordinates: 1770800..1771204 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: JN138_RS08320
• Protein ID: WP_009935474.1
• Coordinates: 1770591..1770794 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN138_RS08290	1765996..1766376	+	381	WP_003407768.1	fumarate reductase subunit C	-
JN138_RS08295	1766373..1766750	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
JN138_RS08300	1766818..1767426	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
JN138_RS08305	1767610..1768758	+	1149	Protein_1638	MMPL family transporter	-
JN138_RS08310	1768768..1769214	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
JN138_RS08315	1769249..1770538	+	1290	WP_016719220.1	threonine ammonia-lyase	-
JN138_RS08320	1770591..1770794	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN138_RS08325	1770800..1771204	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
JN138_RS08330	1771221..1772963	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
JN138_RS08335	1772956..1775253	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T189669 WP_003407786.1 NZ_CP069075:1770800-1771204 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T189669 NZ_CP090916:38973-39239 [Staphylococcus epidermidis]
ATGGCTAAATATAAAATCAACATAAAGAATTCAGCGAAGACAGATTTGAAGAAGATAAAACGTTCTCACTTACAAGAACA
ATTTTTGAAAATCGTAGAAATACTAAAATATGATCCATATCAACAAAGTCAATCCTTTGAAAAATTGCATCCTAAACATT
TAGGACGATACTCAAGACGTATCAATCATCAACATAGAGTTGTCTATACAATTGATGAGGAAAATAAAGAAGTATTAATA
TTAGCAGCTTGGTCTCATTATGAATGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT189669 WP_009935474.1 NZ_CP069075:1770591-1770794 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT189669 NZ_CP090916:38716-38973 [Staphylococcus epidermidis]
ATGAAAAGTAAAACACCAACAGAAGCTCGTAAAGATTTTTATCAGTTATTAAAACGTGTCAATAATGACCATGAACCAGT
ATATATTGAAGGCAAATATGATGAGAGTAATGCTGTTATTATAGGCATGGAAGATTGGCGTAGCATTAATGAAACGATAT
ATCTTGAGGCTACAGGCACAATGGACAAAGTTAGAGAACGTGAGCATGACAACAGTGGTTATACAAACATAGGTGATATT
GATTGGGCTAGACTTTAA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46