Detailed information of TA system TA189663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1244105..1244673
• Replicon: chromosome
• Accession: NZ_CP069075
• Organism: Mycobacterium tuberculosis strain N0004

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JN138_RS05985
• Protein ID: WP_003405865.1
• Coordinates: 1244299..1244673 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN138_RS05980
• Protein ID: WP_003405863.1
• Coordinates: 1244105..1244302 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN138_RS05960	1240146..1240784	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN138_RS05965	1240874..1241881	+	1008	WP_015629829.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN138_RS05970	1241898..1242881	-	984	WP_003898731.1	hypothetical protein	-
JN138_RS05975	1242944..1244017	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JN138_RS05980	1244105..1244302	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN138_RS05985	1244299..1244673	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN138_RS05990	1244876..1245574	+	699	WP_016719357.1	hypothetical protein	-
JN138_RS05995	1245692..1245877	+	186	WP_003901093.1	hypothetical protein	-
JN138_RS06000	1245804..1246079	-	276	WP_003405867.1	hypothetical protein	-
JN138_RS06005	1246322..1246645	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN138_RS06010	1246660..1247358	-	699	WP_031646953.1	hypothetical protein	-
JN138_RS06015	1247392..1247601	+	210	WP_003911400.1	hypothetical protein	-
JN138_RS06020	1247553..1248323	-	771	Protein_1186	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T189663 WP_003405865.1 NZ_CP069075:1244299-1244673 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189663 NZ_CP090915:811285-811647 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189663 WP_003405863.1 NZ_CP069075:1244105-1244302 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189663 NZ_CP090915:811118-811288 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07