Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 761685..762477 Replicon chromosome
Accession NZ_CP069075
Organism Mycobacterium tuberculosis strain N0004

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag JN138_RS03535 Protein ID WP_003403386.1
Coordinates 762040..762477 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag JN138_RS03530 Protein ID WP_003403381.1
Coordinates 761685..761930 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN138_RS03500 756704..758210 + 1507 Protein_691 carotenoid cleavage oxygenase -
JN138_RS03505 758291..759301 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
JN138_RS03510 759689..760072 - 384 WP_015629650.1 hypothetical protein -
JN138_RS03515 760167..760322 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
JN138_RS03520 760398..761114 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
JN138_RS03525 761390..761698 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
JN138_RS03530 761685..761930 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
JN138_RS03535 762040..762477 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
JN138_RS03540 762474..762728 - 255 WP_003911263.1 antitoxin VapB7 -
JN138_RS03545 762842..765205 + 2364 WP_003403397.1 arylsulfatase AtsD -
JN138_RS03550 765268..765594 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN138_RS03555 765506..765844 + 339 WP_003403405.1 PIN domain-containing protein -
JN138_RS03560 765841..766014 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T189657 WP_003403386.1 NZ_CP069075:c762477-762040 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T189657 NZ_CP090910:102856-103143 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT189657 WP_003403381.1 NZ_CP069075:c761930-761685 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT189657 NZ_CP090910:102617-102856 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References