Detailed information of TA system TA189655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 759689..760322
• Replicon: chromosome
• Accession: NZ_CP069075
• Organism: Mycobacterium tuberculosis strain N0004

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JN138_RS03510
• Protein ID: WP_015629650.1
• Coordinates: 759689..760072 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: JN138_RS03515
• Protein ID: WP_003403368.1
• Coordinates: 760167..760322 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN138_RS03485	754980..755516	+	537	WP_003403341.1	50S ribosomal protein L10	-
JN138_RS03490	755553..755945	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
JN138_RS03495	755938..756633	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
JN138_RS03500	756704..758210	+	1507	Protein_691	carotenoid cleavage oxygenase	-
JN138_RS03505	758291..759301	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
JN138_RS03510	759689..760072	-	384	WP_015629650.1	hypothetical protein	Toxin
JN138_RS03515	760167..760322	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN138_RS03520	760398..761114	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
JN138_RS03525	761390..761698	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
JN138_RS03530	761685..761930	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
JN138_RS03535	762040..762477	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
JN138_RS03540	762474..762728	-	255	WP_003911263.1	antitoxin VapB7	-
JN138_RS03545	762842..765205	+	2364	WP_003403397.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13886.68 Da
• Isoelectric Point: 6.0803

>T189655 WP_015629650.1 NZ_CP069075:c760072-759689 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRLIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T189655 NZ_CP090910:c29463-28978 [Enterobacter hormaechei]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT189655 WP_003403368.1 NZ_CP069075:c760322-760167 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT189655 NZ_CP090910:c29717-29451 [Enterobacter hormaechei]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSF8