Detailed information of TA system TA189650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 703327..703973
• Replicon: chromosome
• Accession: NZ_CP069075
• Organism: Mycobacterium tuberculosis strain N0004

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: JN138_RS03185
• Protein ID: WP_003403137.1
• Coordinates: 703327..703740 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: JN138_RS03190
• Protein ID: WP_003403139.1
• Coordinates: 703737..703973 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN138_RS03165	699410..700960	+	1551	WP_003403119.1	MlaD family protein	-
JN138_RS03170	701012..701404	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
JN138_RS03175	701401..701658	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
JN138_RS03180	701841..703076	-	1236	WP_003403128.1	ATP-binding protein	-
JN138_RS03185	703327..703740	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN138_RS03190	703737..703973	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
JN138_RS03195	704077..704583	-	507	WP_003900973.1	two component system sensor kinase HK1	-
JN138_RS03200	704697..705227	-	531	WP_003917320.1	two component system sensor kinase HK2	-
JN138_RS03205	705211..705906	-	696	WP_003918099.1	two-component system response regulator TcrA	-
JN138_RS03210	706029..706340	+	312	WP_003403164.1	hypothetical protein	-
JN138_RS03215	706412..707362	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
JN138_RS03220	707603..708187	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
JN138_RS03225	708189..708899	+	711	Protein_639	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T189650 WP_003403137.1 NZ_CP069075:c703740-703327 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T189650 NZ_CP090909:2761187-2761501 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTACACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATCTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT189650 WP_003403139.1 NZ_CP069075:c703973-703737 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT189650 NZ_CP090909:2761498-2761941 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTGT
GCGCCAGAATTAGCCGTTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCTGTTCCTGTCGGA
ATTGCAGTTTTAAGAACATTGATGGATCAATACAATCTGACGATATCGGATTTTCAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGTCAGATTTGGGGTATCAC
CTGCGCTATTTATTGAGTGTCACCACCCAACAAACGAAACGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89