Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3207763..3208450 Replicon chromosome
Accession NZ_CP069074
Organism Mycobacterium tuberculosis strain N1274

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag JN139_RS15310 Protein ID WP_003414624.1
Coordinates 3208007..3208450 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag JN139_RS15305 Protein ID WP_003414620.1
Coordinates 3207763..3208020 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN139_RS15285 3203083..3203937 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
JN139_RS15290 3203993..3205156 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
JN139_RS15295 3205173..3206387 - 1215 WP_003414610.1 zinc metalloprotease Rip -
JN139_RS15300 3206395..3207636 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
JN139_RS15305 3207763..3208020 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
JN139_RS15310 3208007..3208450 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
JN139_RS15315 3208530..3209192 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
JN139_RS15320 3209288..3209479 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
JN139_RS15325 3209811..3211559 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
JN139_RS15330 3211655..3212236 + 582 WP_003414644.1 fasciclin domain-containing protein -
JN139_RS15335 3212336..3212602 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T189636 WP_003414624.1 NZ_CP069074:3208007-3208450 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T189636 NZ_CP090908:2028768-2028864 [Serratia marcescens]
AACAAGCCCTGCATTAAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATC
GTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT189636 WP_003414620.1 NZ_CP069074:3207763-3208020 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT189636 NZ_CP090908:c2028864-2028724 [Serratia marcescens]
AAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TTAATGCAGGGCTTGTTCAGCCGTGCACTTTAAGAGTAGCCTACCGCGCCAGTTTTGCCAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References