Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3161246..3161850 Replicon chromosome
Accession NZ_CP069074
Organism Mycobacterium tuberculosis strain N1274

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag JN139_RS15085 Protein ID WP_003414492.1
Coordinates 3161246..3161638 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag JN139_RS15090 Protein ID WP_003414495.1
Coordinates 3161635..3161850 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN139_RS15055 3156396..3157184 - 789 WP_192940011.1 CRISPR-associated endoribonuclease Cas6 -
JN139_RS15060 3157518..3158063 - 546 WP_014584866.1 DUF1802 family protein -
JN139_RS15065 3158335..3159219 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
JN139_RS15070 3159222..3160109 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
JN139_RS15075 3160414..3160959 - 546 WP_019284034.1 DUF1802 family protein -
JN139_RS15080 3160956..3161225 - 270 WP_003414489.1 DUF2277 family protein -
JN139_RS15085 3161246..3161638 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
JN139_RS15090 3161635..3161850 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JN139_RS15095 3161897..3162646 + 750 WP_003414497.1 enoyl-CoA hydratase -
JN139_RS15100 3162725..3163807 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
JN139_RS15105 3163800..3165110 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
JN139_RS15110 3165113..3165940 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
JN139_RS15115 3165937..3166848 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T189634 WP_003414492.1 NZ_CP069074:c3161638-3161246 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T189634 NZ_CP090908:1616557-1616865 [Serratia marcescens]
ATGCAGTTTACCGTATATGACAATACATATCCGGCATCAGCCTATCCCTACTTACTGGACGTTCAAAGCGATCTTATTGA
TGCCCTCTCGACCCGGTTAATGATCCCTCTTTATGCTTTGGATAACGTCAGGGTTAAAATTTCTGCACGCTTATGCCCGG
AGATTGAGGTAAACGGCGAAAAGTTTCTCGTTATGACGCATGAGATGGCTGCCGTACGTATTTCACAGCTCGGGAAGGCC
GTTGGTAATGCCAATGAGCACAGGAATCAAATTAAAGCTGCCATCGATTTTCTGATTGATGGCTTTTGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT189634 WP_003414495.1 NZ_CP069074:c3161850-3161635 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT189634 NZ_CP090908:1616318-1616554 [Serratia marcescens]
ATGGCAAATAACAATGTGACGAAAAAGACAGTCAACGTAACCATCGATCGCGATCTTTTCCAGCGCGCAAAATCCCTGGG
CGTCAATGTGTCATCCGTATTGACGGACGCATTGAGCGTTCGTGTCAGGGACATTGAGATTCAACAGTGGCGCGAGCAAA
ATCGTGCGGCGTTAGAAGAACTTAATCGTATCACTGAAGAAAATGGTTTGTTGTCTGATGAATATCGGACATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References