Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3135042..3135612 Replicon chromosome
Accession NZ_CP069074
Organism Mycobacterium tuberculosis strain N1274

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JN139_RS14940 Protein ID WP_003414166.1
Coordinates 3135042..3135398 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JN139_RS14945 Protein ID WP_003901465.1
Coordinates 3135382..3135612 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN139_RS14920 3130494..3132182 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
JN139_RS14925 3132186..3132512 - 327 WP_003414157.1 hypothetical protein -
JN139_RS14930 3132685..3133272 + 588 WP_003914429.1 DUF3558 family protein -
JN139_RS14935 3133291..3134940 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
JN139_RS14940 3135042..3135398 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JN139_RS14945 3135382..3135612 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
JN139_RS14950 3135655..3136698 - 1044 WP_016720604.1 DUF2293 domain-containing protein -
JN139_RS14955 3136697..3137164 + 468 WP_003414177.1 DUF1778 domain-containing protein -
JN139_RS14960 3137340..3137594 - 255 WP_003917684.1 hypothetical protein -
JN139_RS14965 3137742..3138146 + 405 WP_003414181.1 hypothetical protein -
JN139_RS14970 3138143..3138334 + 192 WP_003414184.1 hypothetical protein -
JN139_RS14975 3138551..3138811 + 261 Protein_2954 transposase -
JN139_RS14980 3139921..3140178 + 258 WP_003899489.1 hypothetical protein -
JN139_RS14985 3140283..3140594 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T189632 WP_003414166.1 NZ_CP069074:c3135398-3135042 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T189632 NZ_CP090908:502847-503122 [Serratia marcescens]
ATGCGTATCGAATGGGATAATGCAGCGCTACGGGACAGGGAACGTATTTTTGAGTTTCTTTATATTTTCAATCCACAGGC
GGCAGTGCAGGCTGATAACGAGATAGATAAAGCGGTAAAGCGCCTGCTGGATCATCCTGAATTAGGTAAGCCTTGGTATG
GGAAGGCACGTAAGCTGCTAGTTAACAAGGCCTCCCTGTTGCTGGTGTATATCATCGTCGATGATGTTATTAAGGTTTTC
TCGATTGCCCACCAGCGCGAGAAATTTCCAAACTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT189632 WP_003901465.1 NZ_CP069074:c3135612-3135382 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT189632 NZ_CP090908:502551-502838 [Serratia marcescens]
ATGGAAAGTCGCATCCAGTTTCGCATCGAAGATGAAACCAAGCGTTTGGCGCAAAAGGCCGCGGAAGCTAAAGGAACGAC
ATTGAGTGAAGCCTGCCGCCGCTTGGCGGAGCAAATGGCTGATGAGCAACGGGCCGCGGAGGAGCACGAAAATTGGTTGA
AAGATAAGGTGGATGCAGCTTTTTCCCGTCTGCATGAGGGTAATGCTGTCTACCTTAGCCAACAAGAAGTAGAAGAAAGC
ATGGATGCCTTTAAGGCAAAAATTAGAGCGAAATACGACCGAAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References