Detailed information of TA system TA189631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3095458..3096145
• Replicon: chromosome
• Accession: NZ_CP069074
• Organism: Mycobacterium tuberculosis strain N1274

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: JN139_RS14720
• Protein ID: WP_003414064.1
• Coordinates: 3095750..3096145 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN139_RS14715
• Protein ID: WP_003414061.1
• Coordinates: 3095458..3095724 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN139_RS14685	3091097..3091999	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN139_RS14690	3092068..3092820	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN139_RS14700	3093064..3093339	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN139_RS14705	3093336..3094958	-	1623	WP_015630537.1	class I SAM-dependent DNA methyltransferase	-
JN139_RS14710	3095045..3095461	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
JN139_RS14715	3095458..3095724	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN139_RS14720	3095750..3096145	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN139_RS14725	3096142..3096411	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN139_RS14730	3096421..3097515	-	1095	WP_015630539.1	restriction endonuclease subunit S	-
JN139_RS14735	3097512..3097931	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN139_RS14740	3097930..3098004	+	75	Protein_2907	hypothetical protein	-
JN139_RS14745	3098005..3098484	-	480	WP_003414073.1	dihydrofolate reductase	-
JN139_RS14750	3098555..3099355	-	801	WP_003911953.1	thymidylate synthase	-
JN139_RS14755	3099511..3100248	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T189631 WP_003414064.1 NZ_CP069074:c3096145-3095750 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T189631 NZ_CP090908:84604-84972 [Serratia marcescens]
ATGCAGACCTTACCTGTCACCCCGAAACGGGCGGCTTACGCTTGCCCATCACCCGTTGTTGTCTGGCAATCCATGCTCAC
GTATCTGCTGGAGCAGCATTACGGCTTGGCGCTTAACGATACCGAGTTCAGTGATGACACCGTTATCCATGAACACATTG
ACGCCGGGATCTCTCTGGCTGATGCGTTGAATTTCACAGTGGAAAAGTTTGGCCTGGTTCGCACCGATCGCAGGGGGTTC
TCCTGTCAGGAACAATCCCCTTTTATCACGGCTATCGATATTCTCCGTGCCCGTCGAGCTACCGGCCTGATGACCCGTAT
GGGTTACCAGACCATCACCTCAGTTATCCGGGGAGAAAAGCAGGCATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189631 WP_003414061.1 NZ_CP069074:c3095724-3095458 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189631 NZ_CP090908:84206-84541 [Serratia marcescens]
ATGTCATCAAAAACTACCCCAGCCTGGGGACTTAAACGCAACGTCACTCCGCAGTTTGGTGCCCGCCTTGTACAAGAAGG
CGACCGGCTGCATTTTCTGGCCGACCGTGCCGATATCAGCGGGAAATTCAGTGACGAGCAGGTACGTGATTTAAATAACG
CCTTCCCGCAGTTTATCAACCACCTGGAACTGATGCTGCTCTCCGGTGAACTCAATCCACGTTATCAACACTGCGTCACG
CTCTACCACAAGGGACTAATCTGTGAAGCCGATAGTCTGGGTTCACACGGTTATGTGTATCTCGCCATTTATCCTACACC
CCAATCAACAGCATAA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64