Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2895656..2896293 Replicon chromosome
Accession NZ_CP069074
Organism Mycobacterium tuberculosis strain N1274

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN139_RS13620 Protein ID WP_003413180.1
Coordinates 2895656..2896051 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN139_RS13625 Protein ID WP_003413183.1
Coordinates 2896048..2896293 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN139_RS13580 2891059..2892270 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
JN139_RS13585 2892397..2893056 + 660 WP_003900846.1 LppA family lipoprotein -
JN139_RS13590 2893053..2893715 + 663 WP_023643170.1 LppA family lipoprotein -
JN139_RS13595 2893712..2893990 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN139_RS13600 2894083..2894496 + 414 WP_003413164.1 PIN domain nuclease -
JN139_RS13605 2894535..2894792 + 258 WP_003413167.1 CopG family transcriptional regulator -
JN139_RS13610 2894789..2895166 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN139_RS13615 2895182..2895556 - 375 WP_003413177.1 hypothetical protein -
JN139_RS13620 2895656..2896051 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN139_RS13625 2896048..2896293 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN139_RS13630 2896704..2897123 - 420 WP_003413190.1 A24 family peptidase -
JN139_RS13635 2897135..2897944 - 810 WP_003413193.1 shikimate dehydrogenase -
JN139_RS13640 2897941..2899194 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN139_RS13645 2899187..2899699 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189627 WP_003413180.1 NZ_CP069074:c2896051-2895656 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189627 NZ_CP090905:472963-473313 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189627 WP_003413183.1 NZ_CP069074:c2896293-2896048 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189627 NZ_CP090905:472676-472957 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References