Detailed information of TA system TA189625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2894083..2894792
• Replicon: chromosome
• Accession: NZ_CP069074
• Organism: Mycobacterium tuberculosis strain N1274

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ26
• Locus tag: JN139_RS13600
• Protein ID: WP_003413164.1
• Coordinates: 2894083..2894496 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: JN139_RS13605
• Protein ID: WP_003413167.1
• Coordinates: 2894535..2894792 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN139_RS13570	2889136..2890341	-	1206	WP_003413027.1	chorismate synthase	-
JN139_RS13575	2890449..2890763	+	315	WP_009937839.1	hypothetical protein	-
JN139_RS13580	2891059..2892270	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
JN139_RS13585	2892397..2893056	+	660	WP_003900846.1	LppA family lipoprotein	-
JN139_RS13590	2893053..2893715	+	663	WP_023643170.1	LppA family lipoprotein	-
JN139_RS13595	2893712..2893990	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
JN139_RS13600	2894083..2894496	+	414	WP_003413164.1	PIN domain nuclease	Toxin
JN139_RS13605	2894535..2894792	+	258	WP_003413167.1	CopG family transcriptional regulator	Antitoxin
JN139_RS13610	2894789..2895166	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
JN139_RS13615	2895182..2895556	-	375	WP_003413177.1	hypothetical protein	-
JN139_RS13620	2895656..2896051	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
JN139_RS13625	2896048..2896293	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
JN139_RS13630	2896704..2897123	-	420	WP_003413190.1	A24 family peptidase	-
JN139_RS13635	2897135..2897944	-	810	WP_003413193.1	shikimate dehydrogenase	-
JN139_RS13640	2897941..2899194	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
JN139_RS13645	2899187..2899699	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15045.00 Da
• Isoelectric Point: 5.4673

>T189625 WP_003413164.1 NZ_CP069074:2894083-2894496 [Mycobacterium tuberculosis]
MVFCVDTSAWHHAARPEVARRWLAALSADQIGICDHVRLEILYSANSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTIVWHYDENYDRVAAITGQPTEWIVPRGTL

Nucleotide

• Length: 414 bp

>T189625 NZ_CP090898:755839-756174 [Synechococcus sp. NB0720_010]
ATGGTGAGCGCACCGGAACGCGGTGACTTGATCTGGCTGAGCTTCACGCCTCAGCAAGGCAGAGAGCAGGCTGGGCGCAG
GCCAGCACTGGTGATCTCACCGGCGGCCTACAACAGCAAGACAGGCCTCGCCCTGGTCTGCCCAATCACCAGCAAAGCGA
AGGGTTACCCGTTCGAAGTGGTTCTGCCGAATCAGGGAACCGTGCAAGGCGTTGTCCTGACGGATCAACTCAGAAGCCTC
GACTGGCGTGCTCGCCAGGCCGAACGCATTGAAAAGTCTCCGCCTGATGTCGTGGACCAGGCCGTGAAGCTCATCGGAAC
CCTCCTGACCTCCTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT189625 WP_003413167.1 NZ_CP069074:2894535-2894792 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT189625 NZ_CP090898:755609-755845 [Synechococcus sp. NB0720_010]
ATGCGGGTCAAGGTTCAAGCGTGGGGAAACAGTCTCGGGCTACGCATCCCCAAGGCCTACGCCAGCGAACTCGGAGTGAG
GGCAGGCAGTGAGATGGAAGTCAGCGTCGAGGCGGGAGCCTTGCGTGCTGAGCTGGCCCCTCCCGCTCAACTTGACGCGC
TGCTGGCAAAGATCACCGACGAGAACCGCCATGACCAGATCGAGTGGGGCGAGCCCTCTGGAGGCGAGACATGGTGA

Structures

Toxin

• AlphaFold DB: G0TQ26

Antitoxin

• AlphaFold DB: A0A829C9W5