Detailed information of TA system TA189624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2880196..2880836
• Replicon: chromosome
• Accession: NZ_CP069074
• Organism: Mycobacterium tuberculosis strain N1274

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: JN139_RS13515
• Protein ID: WP_003412970.1
• Coordinates: 2880196..2880615 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: JN139_RS13520
• Protein ID: WP_003412975.1
• Coordinates: 2880612..2880836 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN139_RS13485	2875781..2876503	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
JN139_RS13490	2877020..2877247	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
JN139_RS13495	2877244..2877645	+	402	WP_003412963.1	PIN domain-containing protein	-
JN139_RS13500	2877680..2878600	-	921	WP_003412965.1	restriction endonuclease	-
JN139_RS13505	2878941..2879147	-	207	WP_229298035.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JN139_RS13510	2879245..2880195	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
JN139_RS13515	2880196..2880615	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN139_RS13520	2880612..2880836	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
JN139_RS13525	2880867..2883710	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
JN139_RS13530	2883782..2884183	-	402	WP_003412981.1	hypothetical protein	-
JN139_RS13535	2884183..2884653	-	471	WP_003899364.1	transcription antitermination factor NusB	-
JN139_RS13540	2884656..2885219	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T189624 WP_003412970.1 NZ_CP069074:c2880615-2880196 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T189624 NZ_CP090898:728720-728992 [Synechococcus sp. NB0720_010]
ATGCCGAGCCTTGAACGACTCAGCTGGACCACTGCGGCCTGGGGCGACTACCTCTACTGGCAGGGACAGGACCGCAAAAC
CCTTAGGCGCATCAACCAACTGATCCAGGCCTGCCTGAGGCACCCGTTTGAGGGCATTGGCAAGCCAGAGCCCCTCAGGG
AATCCCTCTCAGGTTGCTGGTCCAGGCGCATCGATGCAGAGCATCGGCTCGTCTACCGCGTCGACGGCCCTCTCCTGGTG
ATCCTGGCCTGCCGCTACCACTACCGCGTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT189624 WP_003412975.1 NZ_CP069074:c2880836-2880612 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT189624 NZ_CP090898:728473-728733 [Synechococcus sp. NB0720_010]
ATGCAGGTGGTCAGCTTTTCAGAAGCGCGCCAGAGCTTCAAAGCCGTCCTGGATCGAGTCGAGGCCGATGCGGACGTCAC
CTTGATCACCCGGCGGGATGCCAGCAGCGCCGTCCTGATGTCCCTCGAGACCTACAACAGCCTGATGGAGACCGTGCACC
TGCTGCGCAGTCCCGCCAACGCCGCCCACCTGCAACAGTCCCTCGAGCAAGCCGACCGCGGGGAGTTGGTCGATCACGCA
TTGATCGATGCCGAGCCTTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09