Detailed information of TA system TA189617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2254950..2255536
• Replicon: chromosome
• Accession: NZ_CP069074
• Organism: Mycobacterium tuberculosis strain N1274

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLY3
• Locus tag: JN139_RS10610
• Protein ID: WP_003410010.1
• Coordinates: 2254950..2255294 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL58
• Locus tag: JN139_RS10615
• Protein ID: WP_003410014.1
• Coordinates: 2255288..2255536 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN139_RS10570	2250617..2251045	+	429	WP_003409992.1	cellulose-binding protein	-
JN139_RS10575	2251271..2251810	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
JN139_RS10580	2252022..2252099	+	78	Protein_2089	hypothetical protein	-
JN139_RS10585	2252325..2252864	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
JN139_RS10590	2253384..2253944	-	561	WP_003410001.1	RES family NAD+ phosphorylase	-
JN139_RS10595	2253941..2254282	-	342	WP_003410003.1	DUF2384 domain-containing protein	-
JN139_RS10600	2254368..2254625	+	258	WP_003410006.1	hypothetical protein	-
JN139_RS10605	2254526..2254861	-	336	WP_003410009.1	dehydrogenase	-
JN139_RS10610	2254950..2255294	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	Toxin
JN139_RS10615	2255288..2255536	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	Antitoxin
JN139_RS10620	2255636..2257888	-	2253	WP_016719189.1	cation transporter ATPase CptG	-
JN139_RS10625	2257885..2258157	-	273	WP_003410017.1	DUF1490 family protein	-
JN139_RS10630	2258210..2258566	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-
JN139_RS10635	2258723..2259490	+	768	WP_003410019.1	hemerythrin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-38)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12230.12 Da
• Isoelectric Point: 9.8887

>T189617 WP_003410010.1 NZ_CP069074:c2255294-2254950 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Nucleotide

• Length: 345 bp

>T189617 NZ_CP090888:c1589186-1589007 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9290.34 Da
• Isoelectric Point: 4.3757

>AT189617 WP_003410014.1 NZ_CP069074:c2255536-2255288 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Nucleotide

• Length: 249 bp

>AT189617 NZ_CP090888:c1588970-1588518 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• PDB: 5HK3
• PDB: 5HK0
• PDB: 5HKC

Antitoxin

• PDB: 7WJ0
• PDB: 7WNR
• AlphaFold DB: A0A7U4FAW9