Detailed information of TA system TA189603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1244686..1245254
• Replicon: chromosome
• Accession: NZ_CP069074
• Organism: Mycobacterium tuberculosis strain N1274

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JN139_RS05970
• Protein ID: WP_003405865.1
• Coordinates: 1244880..1245254 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN139_RS05965
• Protein ID: WP_003405863.1
• Coordinates: 1244686..1244883 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN139_RS05945	1240727..1241365	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN139_RS05950	1241455..1242462	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN139_RS05955	1242479..1243462	-	984	WP_003898731.1	hypothetical protein	-
JN139_RS05960	1243525..1244598	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JN139_RS05965	1244686..1244883	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN139_RS05970	1244880..1245254	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN139_RS05975	1245457..1246155	+	699	WP_016719357.1	hypothetical protein	-
JN139_RS05980	1246273..1246458	+	186	WP_003901093.1	hypothetical protein	-
JN139_RS05985	1246385..1246660	-	276	WP_003405867.1	hypothetical protein	-
JN139_RS05990	1246903..1247226	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN139_RS05995	1247241..1247939	-	699	WP_031646953.1	hypothetical protein	-
JN139_RS06000	1247973..1248182	+	210	WP_003911400.1	hypothetical protein	-
JN139_RS06005	1248134..1248904	-	771	Protein_1181	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T189603 WP_003405865.1 NZ_CP069074:1244880-1245254 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189603 NZ_CP090885:c1149437-1149183 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAATCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189603 WP_003405863.1 NZ_CP069074:1244686-1244883 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189603 NZ_CP090885:c1149681-1149439 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07