Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 759772..760564 Replicon chromosome
Accession NZ_CP069074
Organism Mycobacterium tuberculosis strain N1274

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag JN139_RS03515 Protein ID WP_003403386.1
Coordinates 760127..760564 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag JN139_RS03510 Protein ID WP_003403381.1
Coordinates 759772..760017 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN139_RS03480 754792..756297 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
JN139_RS03485 756378..757388 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
JN139_RS03490 757776..758159 - 384 WP_031747368.1 ribonuclease -
JN139_RS03495 758254..758409 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
JN139_RS03500 758485..759201 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
JN139_RS03505 759477..759785 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
JN139_RS03510 759772..760017 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
JN139_RS03515 760127..760564 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
JN139_RS03520 760561..760815 - 255 WP_003911263.1 antitoxin VapB7 -
JN139_RS03525 760929..763292 + 2364 WP_003403397.1 arylsulfatase AtsD -
JN139_RS03530 763355..763681 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
JN139_RS03535 763593..763931 + 339 WP_003403405.1 PIN domain-containing protein -
JN139_RS03540 763928..764101 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T189596 WP_003403386.1 NZ_CP069074:c760564-760127 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T189596 NZ_CP090883:c1611676-1611497 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTAAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT189596 WP_003403381.1 NZ_CP069074:c760017-759772 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT189596 NZ_CP090883:c1611460-1611008 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGCTAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References