Detailed information of TA system TA189587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 681968..682587
• Replicon: chromosome
• Accession: NZ_CP069074
• Organism: Mycobacterium tuberculosis strain N1274

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O53779
• Locus tag: JN139_RS03085
• Protein ID: WP_003403047.1
• Coordinates: 682180..682587 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O53778
• Locus tag: JN139_RS03080
• Protein ID: WP_003403039.1
• Coordinates: 681968..682183 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN139_RS03070	680496..681254	+	759	WP_003898511.1	Mut7-C RNAse domain-containing protein	-
JN139_RS03075	681383..681874	-	492	WP_003403017.1	mycobacterial cell wall protein Rv0580c	-
JN139_RS03080	681968..682183	+	216	WP_003403039.1	type II toxin-antitoxin system antitoxin VapB26	Antitoxin
JN139_RS03085	682180..682587	+	408	WP_003403047.1	type II toxin-antitoxin system toxin ribonuclease C26	Toxin
JN139_RS03090	682647..683333	-	687	WP_003403052.1	LpqN/LpqT family lipoprotein	-
JN139_RS03095	683487..686120	+	2634	WP_003403055.1	GH92 family glycosyl hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-39)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14471.43 Da
• Isoelectric Point: 4.4493

>T189587 WP_003403047.1 NZ_CP069074:682180-682587 [Mycobacterium tuberculosis]
VIIDTSALLAYFDAAEPDHAAVSECIDSSADALVVSPYVVAELDYLVATRVGVDAELAVLRELAGGAWELANCGAAEIEQ
AARIVTKYQDQRIGIADAANVVLADRYRTRTILTLDRRHFSALRPIGGGRFTVIP

Nucleotide

• Length: 408 bp

>T189587 NZ_CP090881:c1176980-1176726 [Streptococcus pneumoniae]
TTGTATAAATTAGTTCCAACAAGACGTTTCATCAAGCAATTGAAAAAATTGGACCGTTATACGCAGAAGCTAATTACAAA
CTATTTACAAATCAATGTTTTGGAAGACCCAAGACGACACGGAAAGGCTTTGGTTGGTAATCGCGTTGGTCAATGGCGCT
ATAGAATTGGTAATTATCGAGTTATCGTACAAATTGTAGATGATGAATTAGTTGTTGCTACTCTAGAAGTTGGTCATCGG
AGAGATATTTATTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7572.66 Da
• Isoelectric Point: 10.5454

>AT189587 WP_003403039.1 NZ_CP069074:681968-682183 [Mycobacterium tuberculosis]
VDKTTVYLPDELKAAVKRAARQRGVSEAQVIRESIRAAVGGAKPPPRGGLYAGSEPIARRVDELLAGFGER

Nucleotide

• Length: 216 bp

>AT189587 NZ_CP090881:c1177224-1176982 [Streptococcus pneumoniae]
ATGACTACTATTACATTAAAAGTTTCTGAAGCTGATAAAACATTTATGAAAGCAATGGCTAAGTTTGAAGGAGTTTCCCT
TTCGGAACTTATTCGCACCAAAACTCTTGAAGCCCTAGAAGATGAATACGATGCTCGTGTGGCAGATTTAGCCTATCAAG
AGTATTTAGAAGACTTGGAAAAAGGAGTTGAACCCATTACTTGGGAAGAAATGATGCATGATTTAGGCTTGAAGGATGAA
TAA

Structures

Toxin

• PDB: 5X3T

Antitoxin

• AlphaFold DB: A0A7U4BSA2