Detailed information of TA system TA189583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 366430..367001
• Replicon: chromosome
• Accession: NZ_CP069074
• Organism: Mycobacterium tuberculosis strain N1274

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: JN139_RS01595
• Protein ID: WP_015629470.1
• Coordinates: 366430..366732 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: JN139_RS01600
• Protein ID: WP_003401563.1
• Coordinates: 366780..367001 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN139_RS01575	361899..362702	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
JN139_RS01580	362712..364109	-	1398	WP_003401544.1	sulfatase	-
JN139_RS01585	364288..366063	+	1776	WP_010886074.1	PE family protein	-
JN139_RS01590	366206..366433	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
JN139_RS01595	366430..366732	+	303	WP_015629470.1	toxin-antitoxin system toxin	Toxin
JN139_RS01600	366780..367001	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
JN139_RS01605	366998..367423	+	426	WP_003401566.1	PIN domain nuclease	-
JN139_RS01610	367559..368191	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
JN139_RS01615	368188..369096	+	909	WP_003900117.1	protochlorophyllide reductase	-
JN139_RS01620	369104..369694	-	591	WP_229298012.1	hypothetical protein	-
JN139_RS01625	369687..369737	-	51	Protein_321	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10472.19 Da
• Isoelectric Point: 4.9609

>T189583 WP_015629470.1 NZ_CP069074:366430-366732 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRATDTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T189583 NZ_CP090879:1916801-1916977 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT189583 WP_003401563.1 NZ_CP069074:366780-367001 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT189583 NZ_CP090879:c1917139-1916965 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48