Detailed information of TA system TA189575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3195980..3196667
• Replicon: chromosome
• Accession: NZ_CP069073
• Organism: Mycobacterium tuberculosis strain N0054

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: JN140_RS15285
• Protein ID: WP_003414624.1
• Coordinates: 3196224..3196667 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: JN140_RS15280
• Protein ID: WP_031647372.1
• Coordinates: 3195980..3196237 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN140_RS15260	3191300..3192154	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
JN140_RS15265	3192210..3193373	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
JN140_RS15270	3193390..3194604	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
JN140_RS15275	3194612..3195853	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
JN140_RS15280	3195980..3196237	+	258	WP_031647372.1	ribbon-helix-helix domain-containing protein	Antitoxin
JN140_RS15285	3196224..3196667	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN140_RS15290	3196747..3197409	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
JN140_RS15295	3197505..3197696	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
JN140_RS15300	3198028..3199776	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
JN140_RS15305	3199872..3200453	+	582	WP_003414644.1	fasciclin domain-containing protein	-
JN140_RS15310	3200553..3200819	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T189575 WP_003414624.1 NZ_CP069073:3196224-3196667 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T189575 NZ_CP090879:c292603-292352 [Staphylococcus aureus]
ATGAATAATCGCGAAAAAATCGAACAGTCCGTTATTAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGGTTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTATTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGGA
AATGACTATTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8960.17 Da
• Isoelectric Point: 10.2193

>AT189575 WP_031647372.1 NZ_CP069073:3195980-3196237 [Mycobacterium tuberculosis]
VRTTIRIDDELYCEVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT189575 NZ_CP090879:c292362-292189 [Staphylococcus aureus]
ATGACTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA

Structures

Toxin

• PDB: 7VWO