Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3149516..3150120 Replicon chromosome
Accession NZ_CP069073
Organism Mycobacterium tuberculosis strain N0054

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag JN140_RS15060 Protein ID WP_003414492.1
Coordinates 3149516..3149908 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag JN140_RS15065 Protein ID WP_003414495.1
Coordinates 3149905..3150120 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN140_RS15030 3144666..3145454 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
JN140_RS15035 3145788..3146333 - 546 WP_014584866.1 DUF1802 family protein -
JN140_RS15040 3146605..3147489 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
JN140_RS15045 3147492..3148379 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
JN140_RS15050 3148684..3149229 - 546 WP_019284034.1 DUF1802 family protein -
JN140_RS15055 3149226..3149495 - 270 WP_003414489.1 DUF2277 family protein -
JN140_RS15060 3149516..3149908 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
JN140_RS15065 3149905..3150120 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JN140_RS15070 3150167..3150916 + 750 WP_003414497.1 enoyl-CoA hydratase -
JN140_RS15075 3150995..3152077 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
JN140_RS15080 3152070..3153380 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
JN140_RS15085 3153383..3154210 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
JN140_RS15090 3154207..3155118 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 3130971..3151373 20402


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T189573 WP_003414492.1 NZ_CP069073:c3149908-3149516 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T189573 NZ_CP090877:2215362-2215514 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT189573 WP_003414495.1 NZ_CP069073:c3150120-3149905 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT189573 NZ_CP090877:2214738-2215337 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References