Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3121786..3122356 Replicon chromosome
Accession NZ_CP069073
Organism Mycobacterium tuberculosis strain N0054

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag JN140_RS14900 Protein ID WP_003414166.1
Coordinates 3121786..3122142 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag JN140_RS14905 Protein ID WP_003901465.1
Coordinates 3122126..3122356 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN140_RS14880 3117238..3118926 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
JN140_RS14885 3118930..3119256 - 327 WP_003414157.1 hypothetical protein -
JN140_RS14890 3119429..3120016 + 588 WP_003914429.1 DUF3558 family protein -
JN140_RS14895 3120035..3121684 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
JN140_RS14900 3121786..3122142 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
JN140_RS14905 3122126..3122356 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
JN140_RS14910 3122399..3123442 - 1044 WP_016720604.1 DUF2293 domain-containing protein -
JN140_RS14915 3123441..3123908 + 468 WP_003414177.1 DUF1778 domain-containing protein -
JN140_RS14920 3124084..3124338 - 255 WP_155936531.1 hypothetical protein -
JN140_RS14925 3124486..3124890 + 405 WP_003414181.1 hypothetical protein -
JN140_RS14930 3124887..3125078 + 192 WP_003414184.1 hypothetical protein -
JN140_RS14935 3125295..3125555 + 261 Protein_2948 transposase -
JN140_RS14940 3126257..3126430 + 174 WP_244750268.1 hypothetical protein -
JN140_RS14945 3126664..3126738 + 75 Protein_2950 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T189571 WP_003414166.1 NZ_CP069073:c3122142-3121786 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T189571 NZ_CP090877:2037686-2037895 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT189571 WP_003901465.1 NZ_CP069073:c3122356-3122126 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT189571 NZ_CP090877:2037381-2037674 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References