Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3082202..3082889 Replicon chromosome
Accession NZ_CP069073
Organism Mycobacterium tuberculosis strain N0054

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag JN140_RS14680 Protein ID WP_003414064.1
Coordinates 3082494..3082889 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag JN140_RS14675 Protein ID WP_003414061.1
Coordinates 3082202..3082468 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN140_RS14645 3077841..3078743 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
JN140_RS14650 3078812..3079564 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
JN140_RS14660 3079808..3080083 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
JN140_RS14665 3080080..3081702 - 1623 WP_015630537.1 class I SAM-dependent DNA methyltransferase -
JN140_RS14670 3081789..3082205 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
JN140_RS14675 3082202..3082468 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JN140_RS14680 3082494..3082889 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
JN140_RS14685 3082886..3083155 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
JN140_RS14690 3083165..3084259 - 1095 WP_015630539.1 restriction endonuclease subunit S -
JN140_RS14695 3084256..3084675 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
JN140_RS14700 3084674..3084748 + 75 Protein_2901 hypothetical protein -
JN140_RS14705 3084749..3085228 - 480 WP_003414073.1 dihydrofolate reductase -
JN140_RS14710 3085299..3086099 - 801 WP_003911953.1 thymidylate synthase -
JN140_RS14715 3086255..3086992 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T189570 WP_003414064.1 NZ_CP069073:c3082889-3082494 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T189570 NZ_CP090877:1978230-1978334 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT189570 WP_003414061.1 NZ_CP069073:c3082468-3082202 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT189570 NZ_CP090877:c1978492-1978329 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAATGCCGGTCAAAGCGAATAGAAGGTT
ATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TF65

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References