Detailed information of TA system TA189569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3081789..3082468
• Replicon: chromosome
• Accession: NZ_CP069073
• Organism: Mycobacterium tuberculosis strain N0054

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: JN140_RS14670
• Protein ID: WP_003414059.1
• Coordinates: 3081789..3082205 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: JN140_RS14675
• Protein ID: WP_003414061.1
• Coordinates: 3082202..3082468 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN140_RS14645	3077841..3078743	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JN140_RS14650	3078812..3079564	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
JN140_RS14660	3079808..3080083	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
JN140_RS14665	3080080..3081702	-	1623	WP_015630537.1	class I SAM-dependent DNA methyltransferase	-
JN140_RS14670	3081789..3082205	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
JN140_RS14675	3082202..3082468	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN140_RS14680	3082494..3082889	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
JN140_RS14685	3082886..3083155	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
JN140_RS14690	3083165..3084259	-	1095	WP_015630539.1	restriction endonuclease subunit S	-
JN140_RS14695	3084256..3084675	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
JN140_RS14700	3084674..3084748	+	75	Protein_2901	hypothetical protein	-
JN140_RS14705	3084749..3085228	-	480	WP_003414073.1	dihydrofolate reductase	-
JN140_RS14710	3085299..3086099	-	801	WP_003911953.1	thymidylate synthase	-
JN140_RS14715	3086255..3086992	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T189569 WP_003414059.1 NZ_CP069073:c3082205-3081789 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T189569 NZ_CP090877:1718284-1718550 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT189569 WP_003414061.1 NZ_CP069073:c3082468-3082202 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT189569 NZ_CP090877:1718033-1718284 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64