Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2941749..2942463 Replicon chromosome
Accession NZ_CP069073
Organism Mycobacterium tuberculosis strain N0054

Toxin (Protein)


Gene name vapC Uniprot ID G0TQK0
Locus tag JN140_RS13835 Protein ID WP_003413460.1
Coordinates 2942023..2942463 (+) Length 147 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ20
Locus tag JN140_RS13830 Protein ID WP_003413456.1
Coordinates 2941749..2942036 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN140_RS13795 2937172..2937408 + 237 Protein_2725 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JN140_RS13800 2937413..2937817 + 405 WP_003413432.1 type II toxin-antitoxin system VapC family toxin -
JN140_RS13805 2938034..2938654 + 621 WP_003413441.1 DUF4178 domain-containing protein -
JN140_RS13810 2938665..2939159 + 495 WP_003413444.1 DUF2617 family protein -
JN140_RS13815 2939156..2939587 + 432 WP_003899390.1 DUF4247 domain-containing protein -
JN140_RS13820 2939612..2940070 + 459 WP_003413451.1 DUF350 domain-containing protein -
JN140_RS13825 2940067..2941638 + 1572 WP_003899392.1 polyamine aminopropyltransferase -
JN140_RS13830 2941749..2942036 + 288 WP_003413456.1 antitoxin VapB41 Antitoxin
JN140_RS13835 2942023..2942463 + 441 WP_003413460.1 type II toxin-antitoxin system VapC family toxin Toxin
JN140_RS13840 2942484..2943239 - 756 WP_003413464.1 YebC/PmpR family DNA-binding transcriptional regulator -
JN140_RS13845 2943372..2943968 - 597 WP_003413465.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
JN140_RS13850 2943976..2944821 - 846 WP_003413466.1 acyl-CoA thioesterase II -
JN140_RS13855 2944850..2945749 - 900 WP_003413468.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
JN140_RS13860 2945877..2946551 + 675 WP_003413471.1 pyridoxamine 5'-phosphate oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-146)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 147 a.a.        Molecular weight: 16026.38 Da        Isoelectric Point: 6.8599

>T189567 WP_003413460.1 NZ_CP069073:2942023-2942463 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Download         Length: 441 bp

>T189567 NZ_CP090877:1661428-1661694 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATGATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10453.98 Da        Isoelectric Point: 7.2797

>AT189567 WP_003413456.1 NZ_CP069073:2941749-2942036 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Download         Length: 288 bp

>AT189567 NZ_CP090877:1661171-1661428 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQK0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BWB8

References