Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2881412..2882049 Replicon chromosome
Accession NZ_CP069073
Organism Mycobacterium tuberculosis strain N0054

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag JN140_RS13570 Protein ID WP_003413180.1
Coordinates 2881412..2881807 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag JN140_RS13575 Protein ID WP_003413183.1
Coordinates 2881804..2882049 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN140_RS13530 2876815..2878026 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
JN140_RS13535 2878153..2878812 + 660 WP_003900846.1 LppA family lipoprotein -
JN140_RS13540 2878809..2879471 + 663 WP_023643170.1 LppA family lipoprotein -
JN140_RS13545 2879468..2879746 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
JN140_RS13550 2879839..2880252 + 414 WP_003413164.1 PIN domain nuclease -
JN140_RS13555 2880291..2880548 + 258 WP_003413167.1 CopG family transcriptional regulator -
JN140_RS13560 2880545..2880922 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
JN140_RS13565 2880938..2881312 - 375 WP_003413177.1 hypothetical protein -
JN140_RS13570 2881412..2881807 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
JN140_RS13575 2881804..2882049 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
JN140_RS13580 2882460..2882879 - 420 WP_003413190.1 A24 family peptidase -
JN140_RS13585 2882891..2883699 - 809 Protein_2683 shikimate dehydrogenase -
JN140_RS13590 2883696..2884949 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
JN140_RS13595 2884942..2885454 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T189566 WP_003413180.1 NZ_CP069073:c2881807-2881412 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T189566 NZ_CP090877:580542-580730 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT189566 WP_003413183.1 NZ_CP069073:c2882049-2881804 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT189566 NZ_CP090877:580253-580513 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References