Detailed information of TA system TA189557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2261962..2262588
• Replicon: chromosome
• Accession: NZ_CP069073
• Organism: Mycobacterium tuberculosis strain N0054

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: JN140_RS10650
• Protein ID: WP_003410075.1
• Coordinates: 2262190..2262588 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: JN140_RS10645
• Protein ID: WP_003911750.1
• Coordinates: 2261962..2262189 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN140_RS10635	2260001..2260345	-	345	WP_003410065.1	ferredoxin family protein	-
JN140_RS10640	2260534..2261703	-	1170	WP_003899126.1	ATP-binding protein	-
JN140_RS10645	2261962..2262189	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN140_RS10650	2262190..2262588	+	399	WP_003410075.1	PIN domain nuclease	Toxin
JN140_RS10655	2262771..2263202	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
JN140_RS10660	2263303..2263737	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
JN140_RS10665	2264174..2264353	-	180	Protein_2107	hypothetical protein	-
JN140_RS10670	2264441..2265061	+	621	WP_003410086.1	IS110 family transposase	-
JN140_RS10675	2265015..2265605	+	591	WP_003899131.1	IS110 family transposase	-
JN140_RS10680	2265733..2266989	-	1257	WP_031647414.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T189557 WP_003410075.1 NZ_CP069073:2262190-2262588 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T189557 NZ_CP090876:159881-160243 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACATACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT189557 WP_003911750.1 NZ_CP069073:2261962-2262189 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT189557 NZ_CP090876:159714-159884 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8