Detailed information of TA system TA189555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 2236719..2237617
• Replicon: chromosome
• Accession: NZ_CP069073
• Organism: Mycobacterium tuberculosis strain N0054

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: JN140_RS10525
• Protein ID: WP_003410001.1
• Coordinates: 2236719..2237279 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: JN140_RS10530
• Protein ID: WP_003410003.1
• Coordinates: 2237276..2237617 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN140_RS10495	2231888..2232541	-	654	WP_003409976.1	cutinase Cfp21	-
JN140_RS10500	2232656..2232886	+	231	WP_003409980.1	DUF167 domain-containing protein	-
JN140_RS10505	2232971..2233882	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
JN140_RS10510	2233991..2234590	+	600	WP_003409989.1	L-lysine exporter	-
JN140_RS10515	2235006..2235434	+	429	WP_003409992.1	cellulose-binding protein	-
JN140_RS10520	2235660..2236199	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
JN140_RS10525	2236719..2237279	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
JN140_RS10530	2237276..2237617	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
JN140_RS10535	2237703..2237960	+	258	WP_003410006.1	hypothetical protein	-
JN140_RS10540	2237861..2238196	-	336	WP_003410009.1	dehydrogenase	-
JN140_RS10545	2238285..2238629	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
JN140_RS10550	2238623..2238871	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
JN140_RS10555	2238971..2241223	-	2253	WP_016719189.1	cation transporter ATPase CptG	-
JN140_RS10560	2241220..2241492	-	273	WP_003410017.1	DUF1490 family protein	-
JN140_RS10565	2241545..2241901	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T189555 WP_003410001.1 NZ_CP069073:c2237279-2236719 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T189555 NZ_CP090873:2724874-2725062 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT189555 WP_003410003.1 NZ_CP069073:c2237617-2237276 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT189555 NZ_CP090873:2724585-2724845 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• PDB: 6FKG

Antitoxin

• PDB: 6FKG
• AlphaFold DB: A0A7U4FAE4