Detailed information of TA system TA189551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194134..2194837
• Replicon: chromosome
• Accession: NZ_CP069073
• Organism: Mycobacterium tuberculosis strain N0054

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: JN140_RS10240
• Protein ID: WP_003409778.1
• Coordinates: 2194134..2194463 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: JN140_RS10245
• Protein ID: WP_003409780.1
• Coordinates: 2194460..2194837 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN140_RS10220	2190517..2191587	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
JN140_RS10225	2191584..2192099	+	516	WP_003409718.1	flavin reductase family protein	-
JN140_RS10230	2192096..2193157	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
JN140_RS10235	2193154..2193924	+	771	WP_003409775.1	SDR family oxidoreductase	-
JN140_RS10240	2194134..2194463	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
JN140_RS10245	2194460..2194837	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
JN140_RS10250	2194834..2195424	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
JN140_RS10255	2195479..2196843	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
JN140_RS10260	2196998..2197449	-	452	Protein_2025	hypothetical protein	-
JN140_RS10265	2197513..2197914	+	402	WP_244750305.1	hypothetical protein	-
JN140_RS10270	2197907..2198089	-	183	WP_003409870.1	hypothetical protein	-
JN140_RS10275	2198203..2198553	-	351	WP_003409871.1	hypothetical protein	-
JN140_RS10280	2198564..2199466	-	903	WP_003899097.1	hypothetical protein	-
JN140_RS10285	2199487..2199678	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T189551 WP_003409778.1 NZ_CP069073:c2194463-2194134 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T189551 NZ_CP090873:1564707-1564958 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT189551 WP_003409780.1 NZ_CP069073:c2194837-2194460 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT189551 NZ_CP090873:1564948-1565121 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8