Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1954933..1955546 Replicon chromosome
Accession NZ_CP069073
Organism Mycobacterium tuberculosis strain N0054

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag JN140_RS09160 Protein ID WP_003408465.1
Coordinates 1954933..1955322 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag JN140_RS09165 Protein ID WP_003408469.1
Coordinates 1955319..1955546 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN140_RS09125 1950562..1951476 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
JN140_RS09130 1951479..1952309 + 831 WP_003408448.1 cyclase family protein -
JN140_RS09135 1952309..1952659 + 351 WP_003898986.1 cupin domain-containing protein -
JN140_RS09140 1952712..1953530 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
JN140_RS09145 1953544..1954323 + 780 WP_003408460.1 IclR family transcriptional regulator -
JN140_RS09155 1954754..1954885 + 132 Protein_1804 IS3 family transposase -
JN140_RS09160 1954933..1955322 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
JN140_RS09165 1955319..1955546 - 228 WP_003408469.1 antitoxin Antitoxin
JN140_RS09170 1955764..1957248 + 1485 WP_003408473.1 biotin carboxylase -
JN140_RS09175 1957245..1958492 + 1248 WP_003408476.1 serine hydrolase -
JN140_RS09180 1958535..1958954 - 420 WP_003408483.1 hypothetical protein -
JN140_RS09185 1958944..1959654 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T189549 WP_003408465.1 NZ_CP069073:c1955322-1954933 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T189549 NZ_CP090873:1478250-1478612 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT189549 WP_003408469.1 NZ_CP069073:c1955546-1955319 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT189549 NZ_CP090873:1478083-1478253 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References