Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1692434..1693050 Replicon chromosome
Accession NZ_CP069073
Organism Mycobacterium tuberculosis strain N0054

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag JN140_RS07985 Protein ID WP_003407593.1
Coordinates 1692733..1693050 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag JN140_RS07980 Protein ID WP_003900349.1
Coordinates 1692434..1692736 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JN140_RS07970 1688320..1690167 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
JN140_RS07975 1690168..1692420 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
JN140_RS07980 1692434..1692736 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
JN140_RS07985 1692733..1693050 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
JN140_RS07990 1693047..1694051 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
JN140_RS07995 1694104..1695393 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
JN140_RS08000 1695466..1696083 - 618 WP_003407606.1 class I SAM-dependent methyltransferase -
JN140_RS08005 1696297..1696509 - 213 WP_003898900.1 dodecin family protein -
JN140_RS08010 1696570..1696968 + 399 WP_003900351.1 hypothetical protein -
JN140_RS08015 1697013..1698041 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T189547 WP_003407593.1 NZ_CP069073:1692733-1693050 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T189547 NZ_CP090873:1401268-1401372 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT189547 WP_003900349.1 NZ_CP069073:1692434-1692736 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT189547 NZ_CP090873:c1401530-1401367 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAATGCCGGTCAAAGCGAATAGAAGGTT
ATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References