Detailed information of TA system TA189542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1245378..1245946
• Replicon: chromosome
• Accession: NZ_CP069073
• Organism: Mycobacterium tuberculosis strain N0054

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: JN140_RS05990
• Protein ID: WP_003405865.1
• Coordinates: 1245572..1245946 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: JN140_RS05985
• Protein ID: WP_003405863.1
• Coordinates: 1245378..1245575 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN140_RS05965	1241419..1242057	-	639	WP_003898730.1	lipid droplet-associated protein	-
JN140_RS05970	1242147..1243154	+	1008	WP_015629829.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
JN140_RS05975	1243171..1244154	-	984	WP_003898731.1	hypothetical protein	-
JN140_RS05980	1244217..1245290	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
JN140_RS05985	1245378..1245575	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
JN140_RS05990	1245572..1245946	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
JN140_RS05995	1246149..1246847	+	699	WP_016719357.1	hypothetical protein	-
JN140_RS06000	1246965..1247150	+	186	WP_003901093.1	hypothetical protein	-
JN140_RS06005	1247077..1247352	-	276	WP_003405867.1	hypothetical protein	-
JN140_RS06010	1247595..1247918	+	324	WP_003405871.1	putative quinol monooxygenase	-
JN140_RS06015	1247933..1248631	-	699	WP_031646953.1	hypothetical protein	-
JN140_RS06020	1248665..1248874	+	210	WP_003911400.1	hypothetical protein	-
JN140_RS06025	1248826..1249596	-	771	Protein_1186	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T189542 WP_003405865.1 NZ_CP069073:1245572-1245946 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T189542 NZ_CP090870:c901516-901250 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT189542 WP_003405863.1 NZ_CP069073:1245378-1245575 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT189542 NZ_CP090870:c901767-901516 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAGCGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07